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Development of a dry powder insufflation device with application in in vitro cell-based assays in the context of respiratory delivery

2024-06Journal article Open access
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dc.contributor.authorPontes, Jorge Filipe
dc.contributor.authorDiogo, Hermínio P.
dc.contributor.authorConceição, Eusébio
dc.contributor.authorAlmeida, Maria P.
dc.contributor.authorBorges dos Santos, Rui M.
dc.contributor.authorGrenha, Ana
dc.date.accessioned2024-08-30T09:49:02Z
dc.date.available2024-08-30T09:49:02Z
dc.date.issued2024-06
dc.description.abstractResearch on pharmaceutical dry powders has been increasing worldwide, along with increased therapeutic strategies for an application through the pulmonary or the nasal routes. In vitro methodologies and tests that mimic the respiratory environment and the process of inhalation itself are, thus, essential. The literature frequently reports cell-based in vitro assays that involve testing the dry powders in suspension. This experimental setting is not adequate, as both the lung and the nasal cavity are devoid of abundant liquid. However, devices that permit powder insufflation over cells in culture are either scarce or technically complex and expensive, which is not feasible in early stages of research. In this context, this work proposes the development of a device that allows the delivery of dry powders onto cell surfaces, thus simulating inhalation more appropriately. Subsequently, a quartz crystal microbalance (QCM) was used to establish a technique enabling the determination of dry powder deposition profiles. Additionally, the determination of the viability of respiratory cells (A549) after the insufflation of a dry powder using the developed device was performed. In all, a prototype for dry powder insufflation was designed and developed, using 3D printing methods for its production. It allowed the homogenous dispersion of the insufflated powders over a petri dish and a QCM crystal, and a more detailed study on how dry powders disperse over the supports. The device, already protected by a patent, still requires further improvement, especially regarding the method for powder weighing and the efficiency of the insufflation process, which is being addressed. The impact of insufflation of air and of locust bean gum (LBG)-based microparticles revealed absence of cytotoxic effect, as cell viability roughly above 70 % was always determined.eng
dc.description.sponsorshipIMS-LA/P/0056/2020, ALG-01-0246-FEDER-072020
dc.identifier.doi10.1016/j.ejps.2024.106775
dc.identifier.issn0928-0987
dc.identifier.urihttp://hdl.handle.net/10400.1/25808
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationCentro de Química Estrutural
dc.relationCentro de Química Estrutural
dc.relationStudy of the impact of dry powder insufflation over respiratory epithelial cells
dc.relationStudy of the impact of dry powder insufflation over respiratory epithelial cells
dc.relation.ispartofEuropean Journal of Pharmaceutical Sciences
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAerosol
dc.subjectBiorelevance
dc.subjectDry powder
dc.subjectInsufflation
dc.subjectQuartz crystal microbalance
dc.subjectRespiratory delivery
dc.titleDevelopment of a dry powder insufflation device with application in in vitro cell-based assays in the context of respiratory deliveryeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleStudy of the impact of dry powder insufflation over respiratory epithelial cells
oaire.awardTitleStudy of the impact of dry powder insufflation over respiratory epithelial cells
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F137064%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//COVID%2FBD%2F152626%2F2022/PT
oaire.citation.startPage106775
oaire.citation.titleEuropean Journal of Pharmaceutical Sciences
oaire.citation.volume197
oaire.fundingStream6817 - DCRRNI ID
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oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePontes
person.familyNameConceição
person.familyNameBorges dos Santos
person.familyNameGrenha
person.givenNameJorge Filipe
person.givenNameEusébio
person.givenNameRui M.
person.givenNameAna
person.identifier.ciencia-idCB16-5CBD-8B89
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person.identifier.orcid0000-0002-6447-0242
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person.identifier.orcid0000-0002-4837-244X
person.identifier.orcid0000-0002-2136-1396
person.identifier.ridI-7931-2015
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person.identifier.ridH-1392-2017
person.identifier.scopus-author-id57195033461
person.identifier.scopus-author-id6603299150
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person.identifier.scopus-author-id8607930100
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