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Disclosing proteins in the leaves of cork oak plants associated with the immune response to Phytophthora cinnamomi inoculation in the roots: a long-term proteomics approach

2021Journal article Open Access
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dc.contributor.authorCoelho, Ana Cristina
dc.contributor.authorPires, Rosa
dc.contributor.authorSchĂŒtz, Gabriela
dc.contributor.authorSanta, CĂĄtia
dc.contributor.authorManadas, Bruno
dc.contributor.authorPinto, Patricia IS
dc.date.accessioned2021-03-18T15:29:49Z
dc.date.available2021-03-18T15:29:49Z
dc.date.issued2021
dc.description.abstractThe pathological interaction between oak trees and Phytophthora cinnamomi has implications in the cork oak decline observed over the last decades in the Iberian Peninsula. During host colonization, the phytopathogen secretes effector molecules like elicitins to increase disease effectiveness. The objective of this study was to unravel the proteome changes associated with the cork oak immune response triggered by P. cinnamomi inoculation in a long-term assay, through SWATH-MS quantitative proteomics performed in the oak leaves. Using the Arabidopis proteome database as a reference, 424 proteins were confidently quantified in cork oak leaves, of which 80 proteins showed a p-value below 0.05 or a fold-change greater than 2 or less than 0.5 in their levels between inoculated and control samples being considered as altered. The inoculation of cork oak roots with P. cinnamomi increased the levels of proteins associated with protein-DNA complex assembly, lipid oxidation, response to endoplasmic reticulum stress, and pyridine-containing compound metabolic process in the leaves. In opposition, several proteins associated with cellular metabolic compound salvage and monosaccharide catabolic process had significantly decreased abundances. The most significant abundance variations were observed for the Ribulose 1,5-Bisphosphate Carboxylase small subunit (RBCS1A), Heat Shock protein 90-1 (Hsp90-1), Lipoxygenase 2 (LOX2) and Histone superfamily protein H3.3 (A8MRLO/At4G40030) revealing a pertinent role for these proteins in the host-pathogen interaction mechanism. This work represents the first SWATH-MS analysis performed in cork oak plants inoculated with P. cinnamomi and highlights host proteins that have a relevant action in the homeostatic states that emerge from the interaction between the oomycete and the host in the long term and in a distal organ.pt_PT
dc.description.sponsorshipFCT:UID/Multi/00631/2019 and UIDB/00631/2020 CEOT BASE to CEOT and ACC, GS and RP; UIDB/ 04326/2020 to CCMAR; Norma Transitoria- DL57/2016/CP1361/CT0015 to PP; contract NIBAP (ALG-01-0247-FEDER-037303) to RP; projects POCI-01-0145-FEDER-007440 (Ref. UIDB/04539/ 2020), POCI-01-0145-FEDER-016428 (Ref. SAICTPAC/0010/2015), POCI-01-0145- FEDER-029311 (Ref. PTDC/BTM-TEC/29311/ 2017), POCI-01-0145-FEDER-30943 (Ref. PTDC/ MECPSQ/30943/2017) and PTDC/MED-NEU/ 27946/2017 to CNC, BM and CS. The work at CNC was also funded by the National Mass Spectrometry Network (RNEM) under contract POCI-01-0145-FEDER-402-022125pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1371/journal.pone.0245148pt_PT
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/15252
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPublic Library of Sciencept_PT
dc.relationTechnology, Entrepreneurship and Innovation Policy Lab
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAcetoacetyl-coa thiolasept_PT
dc.subjectQuercus-suber l.pt_PT
dc.subjectArabidopsis-thalianapt_PT
dc.subjectFunctional-analysispt_PT
dc.subjectManagement optionspt_PT
dc.subjectDisease resistancept_PT
dc.subjectGlycolate oxidasept_PT
dc.subjectGene-expressionpt_PT
dc.subjectJasmonic acidpt_PT
dc.subjectClp proteasept_PT
dc.titleDisclosing proteins in the leaves of cork oak plants associated with the immune response to Phytophthora cinnamomi inoculation in the roots: a long-term proteomics approachpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTechnology, Entrepreneurship and Innovation Policy Lab
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00631%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Iniciativas Empreendedoras de Investigação no Âmbito do Programa CMU-Portugal/CMUP-ERI%2FTPE%2F0028%2F2013/PT
oaire.citation.issue1pt_PT
oaire.citation.startPagee0245148pt_PT
oaire.citation.titlePLOS ONEpt_PT
oaire.citation.volume16pt_PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
oaire.fundingStreamConcurso para Financiamento de Iniciativas Empreendedoras de Investigação no Âmbito do Programa CMU-Portugal
person.familyNameCoelho
person.familyNamePires
person.familyNameSchĂŒtz
person.familyNamePinto
person.givenNameAna Cristina
person.givenNameRosa
person.givenNameGabriela
person.givenNamePatricia IS
person.identifier643457
person.identifier.ciencia-idBE1B-B52D-1576
person.identifier.ciencia-id9A17-CF27-8DBA
person.identifier.ciencia-id341B-DE9D-AFC7
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.orcid0000-0002-2919-5468
person.identifier.orcid0000-0002-7456-9414
person.identifier.orcid0000-0001-5081-3913
person.identifier.orcid0000-0001-7854-3898
person.identifier.ridO-5305-2015
person.identifier.ridM-3817-2013
person.identifier.scopus-author-id22940342200
person.identifier.scopus-author-id57201364198
person.identifier.scopus-author-id35254562000
person.identifier.scopus-author-id10240774300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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