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The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells
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Resumo(s)
This work explored the medicinal halophyte Frankenia laevis L. (sea heath) as a potential source of bioactive natural products. In this sense, methanol and dichloromethane extracts
were prepared from aerial organs containing flowers, leaves and stems, and were profiled for their
chemical composition using high-performance liquid chromatography coupled with electrospray
ionization mass spectrometry (HPLC-ESI-MS/MS). The extracts were evaluated for their in vitro
antioxidant capacity using five complementary methods: enzyme inhibitory effects on enzymes
related with neurodegeneration (acetyl (AChE) and butyrylcholinesterase (BuChE)), Type 2 diabetes
(α-glucosidase and α-amylase), hyperpigmentation/food oxidation (tyrosinase), and cytotoxicity
towards human hepatocarcinoma (HepG2) cells. Fifty-one molecules were identified in the extracts,
including several derivatives of phenolic acids, lignans and flavonoids, monoterpenes, and hydroxylated derivatives of linoleic acid. The methanol extract was effective in DPPH and ABTS radical
scavenging (EC50 = 0.25 and 0.65 mg/mL, respectively), copper chelation (EC50 = 0.78 mg/mL), and
iron reduction (EC50 = 0.51 mg/mL) activities, whereas the dichloromethane extract had high iron
chelating ability (EC50 = 0.76 mg/mL). Both extracts showed the capacity to inhibit α-glucosidase,
especially the dichloromethane (EC50 = 0.52 mg/mL). This extract also exerted a significant selective
cytotoxicity towards HepG2 cells (EC50 = 52.1 µg/mL, SI > 1.9). In conclusion, extracts from the aerial
parts of sea heath were shown to be a promising source of natural products for pharmaceutical and/or
food additive applications due to their high antioxidant, anti-diabetic, and cytotoxic properties.
Descrição
Palavras-chave
Salt-tolerant plants Frankenia laevis Phenolics Monoterpenes Type 2 diabetes Anti-tumor
Contexto Educativo
Citação
Plants 11 (10): 1353 (2022)
Editora
MDPI