Evaluation of oxidative DNA lesions in plasma and nuclear abnormalities in erythrocytes of wild fish (Liza aurata) as an integrated approach to genotoxicity assessment

Oliveira, M.; Ahmad, I.; Maria, Vera L.; Ferreira, C. S. S.; Serafim, A.; Bebianno, Maria João; Pacheco, M.; Santos, M. A.

http://hdl.handle.net/10400.1/13817

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Abstract(s)

Genetic lesions (8-hydroxy-2'-deoxyguanosine (8-OHdG) and erythrocytic nuclear abnormalities (ENA) were seasonally quantified in the blood of Liza aurata caught at Ria de Aveiro (Portugal), a multi-contaminated aquatic system. Thus, five critical sites were assessed and compared with a reference site (Torreira). Oxidative DNA damage was found in Gafanha (harbour-water area), Laranjo (metal-contaminated) and Vagos (contaminated with PAHs) in the spring; Rio Novo do Principe (near a former paper-mill effluent) in the autumn; Rio Novo do Principe and Vagos in the winter. ENA were higher than Torreira at VAG (spring and winter). Torreira did not display seasonal variation neither in terms of 8-OHdG or total ENA. A positive correlation between 8-OHdG and ENA was found, suggesting oxidative stress as a mechanism involved in the formation of ENA. This study clearly demonstrates the presence of DNA-damaging substances in Ria de Aveiro and recommends the use of 8-OHdG and ENA as biomarkers of environmental contamination.

Keywords

8-Hydroxy-2'-deoxyguanosine Animals Cell nucleus Shape Deoxyguanosine Environmental Monitoring Erythrocytes Micronuclei, Chromosome-Defective Mutagenicity tests Portugal Smegmamorpha Water Pollutants DNA Damage Oxidative Stress Liza aurata Erythrocytic nuclear abnormalities Chemical