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Skin mucus and blood plasma as non-lethal sources of malnutrition protein biomarkers in meagre (Argyrosomus regius)

2025-05Journal article Restricted access
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dc.contributor.authorOliveira, Joana
dc.contributor.authorRaposo de Magalhães, Cláudia
dc.contributor.authorSchrama, Denise
dc.contributor.authorRodrigues, Pedro M.
dc.contributor.authorBarata, Marisa
dc.contributor.authorSoares, Florbela
dc.contributor.authorPousão-Ferreira, Pedro
dc.contributor.authorOliva-Teles, Aires
dc.contributor.authorCouto, Ana
dc.date.accessioned2025-04-12T12:02:17Z
dc.date.available2025-04-12T12:02:17Z
dc.date.issued2025-05
dc.description.abstractDeveloping dietary formulations for aquaculture that meet nutritional requirements is essential to production, as nutrition is key for fish growth and health. However, novel dietary formulations may induce malnutrition, which is complex to evaluate and often requires animal sacrifice. Therefore, finding reliable non-lethal biomarkers to diagnose malnutrition in fish is important. This study aimed to obtain vital information on potential non-lethal biomarkers from blood plasma and skin mucus to assess the fish's nutritional status using meagre (Argyrosomus regius) juveniles. For that purpose, a nutritional challenge was performed with fish fed a fish meal (FM) and fish- oil (FO) based control diet (55.1 % FM; 11 % FO, CTRL), a challenging diet (15 % FM; 7 % FO, CD), and a highly challenging diet (5 % FM; 5 % FO, ED), which, despite being nutritionally complete, may pose digestive and physiological challenges to carnivorous species. Diets significantly affected blood parameters, except for leukocyte counts, peroxidase activity, and immunoglobulin levels. Overall, blood parameters showed potential as non-lethal biomarkers to accurately identify signs of malnutrition. Meagre's plasma and skin mucus proteomes provided crucial information on the species' reaction to malnutrition, and 29 proteins connected to various physiological functions such as metabolism, development and immunity showed potential as non-lethal biomarkers. Significance: The significance of this study lies in the establishment of potential non-lethal biomarkers for diagnosing malnutrition in fish. The results demonstrate that immunological, haematological, and biochemical parameters measured in fish blood can reveal signs of nutritional deficiencies. The findings further highlight that the proteomes of plasma and skin mucus provide valuable information about the fish's nutritional status. Notably, 29 proteins identified in this study, associated with various physiological functions, exhibit biomarker potential and warrant consideration in future research in the field of aquaculture nutrition. Moreover, the research provides critical insights into the proteome of meagre (Argyrosomus regius), enhancing our understanding of the species and contributing to the future improvement of its aquaculture production.eng
dc.description.sponsorshipprojects EMBRC.PT [ALG-01-0145-FEDER-022121] and BIODATA.PT [ALG-01-0145-FEDER-022231]; project INOVAQUA [MAR-021.1.3-FEAMPA-00004]; UI/BD/150902/2021
dc.identifier.doi10.1016/j.jprot.2025.105432
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10400.1/27011
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationUI/BD/150902/2021
dc.relation.ispartofJournal of Proteomics
dc.rights.uriN/A
dc.subjectNutrition
dc.subjectProteins
dc.subjectAquaculture
dc.subjectPhysiology
dc.subjectHealth
dc.titleSkin mucus and blood plasma as non-lethal sources of malnutrition protein biomarkers in meagre (Argyrosomus regius)eng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.startPage105432
oaire.citation.titleJournal of Proteomics
oaire.citation.volume316
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameRaposo de Magalhães
person.familyNameSchrama
person.familyNameRodrigues
person.givenNameCláudia
person.givenNameDenise
person.givenNamePedro M.
person.identifier159154
person.identifier.ciencia-idEB1C-0E40-F003
person.identifier.ciencia-id501C-AC69-68C0
person.identifier.ciencia-id601F-3832-C556
person.identifier.orcid0000-0001-7751-2870
person.identifier.orcid0000-0003-0274-5957
person.identifier.orcid0000-0002-3256-5060
person.identifier.ridH-6511-2016
person.identifier.scopus-author-id55755583400
person.identifier.scopus-author-id55107531400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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