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Presence of Citrus tristeza virus in Angola and São Tomé e Princípe: characterization of isolates based on coat protein gene analysis

dc.contributor.authorSilva, Goncalo
dc.contributor.authorFonseca, Filomena
dc.contributor.authorSantos, C.
dc.contributor.authorNolasco, Gustavo
dc.date.accessioned2015-06-15T14:13:44Z
dc.date.available2015-06-15T14:13:44Z
dc.date.issued2007
dc.description.abstractA first report is given of the presence of Citrus tristeza virus (CTV) in Angola and Sao Tome e Principe. Two out of twenty citrus samples from Sao Tome e Principe and all of the seven samples from Angola were shown by ELISA to contain CTV. The capsid protein gene variants obtained by IC/RT-PCR were characterized by SSCP analysis to test for the presence of different haplotypes in each isolate and nucleotide sequence analysis of each variant leading to a different SSCP pattern, followed by in silico comparison with previously characterized CTV groups. The results confirmed that each isolate could contain different variants, which clustered with the different groups. Variants obtained from each country fitted into the same three clusters.
dc.identifier.issn1125-4653
dc.identifier.otherAUT: GNO00324; FFO00315;
dc.identifier.urihttp://hdl.handle.net/10400.1/6220
dc.language.isoeng
dc.publisherEdizioni ETS, Piza 2007
dc.relation.isbasedonP-004-BJJ
dc.titlePresence of Citrus tristeza virus in Angola and São Tomé e Princípe: characterization of isolates based on coat protein gene analysis
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage152
oaire.citation.startPage149
oaire.citation.titleJournal of Plant Pathology
oaire.citation.volume89
person.familyNameSilva
person.familyNameNolasco
person.givenNameGoncalo
person.givenNameGustavo
person.identifier.orcid0000-0001-5544-2947
person.identifier.orcid0000-0002-7025-6023
person.identifier.scopus-author-id6602625528
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication0e6c622a-5843-4e5c-bc68-96c9206cb452
relation.isAuthorOfPublication87a8035f-bc15-44dd-b8c3-4288c5660fff
relation.isAuthorOfPublication.latestForDiscovery87a8035f-bc15-44dd-b8c3-4288c5660fff

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