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Next-Generation Sequencing (NGS) identified Species-Specific SSR and SNP markers, allow the unequivocal identification of Strawberry Tree (Arbutus unedo L.) germplasm accessions and contribute to assess their genetic relationships

2023-03-31Journal article Open Access
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dc.contributor.authorPereira, Ricardo
dc.contributor.authorAnjos, Isabela Vera Dos
dc.contributor.authorM. Reis, João
dc.contributor.authorDias, Carolina
dc.contributor.authorM Leitão, J
dc.date.accessioned2023-04-13T09:23:41Z
dc.date.available2023-04-13T09:23:41Z
dc.date.issued2023-03-31
dc.date.updated2023-04-12T13:24:19Z
dc.description.abstractThe strawberry tree (Arbutus unedo L.), an evergreen bush to small tree of the Ericaceae family, is a main component of the natural flora of the Mediterranean basin that also grows profusely through the Iberian Peninsula, southwestern France, and Ireland. The small edible red fruits are usually used to produce preserves, jams, and liquors, as the Portuguese “aguardente de medronho”. The leaves and fruits have been used for a long time in traditional medicine, and their bioactive compounds are presently the subject of intense research. A strawberry tree germplasm collection was recently established by the company Corte Velada (Odiáxere, Portugal). A set of 50 germplasm accessions was selected for a breeding program. A next-generation sequencing project was performed, resulting in the establishment of the first strawberry tree genome assembly and further identification of 500 SSR and 500 SNP loci. Individual molecular fingerprints for the unequivocal identification of the selected 50 accessions were established based on 71 markers alleles amplified by 4 SSR and 9 SNP markers. The same species-specific markers alleles combined with 61 random amplified markers amplified by 5 RAPD and 5 ISSR primers were used to assess the genetic variability and genetic relationships among the selected accessions.pt_PT
dc.description.sponsorshipProgram PDR2020; Grant number PDR2020-7.8.4-FEADER042697; LA/P/0121/2020pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPlants 12 (7): 1517 (2023)pt_PT
dc.identifier.doi10.3390/plants12071517pt_PT
dc.identifier.eissn2223-7747
dc.identifier.urihttp://hdl.handle.net/10400.1/19447
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMediterranean Institute for Agriculture, Environment and Development
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectStrawberry treept_PT
dc.subjectArbutus unedo Lpt_PT
dc.subjectPlant germplasm collectionpt_PT
dc.subjectGenome assemblypt_PT
dc.subjectSSR markerspt_PT
dc.subjectSNP markerspt_PT
dc.subjectSNP-CAPS markerspt_PT
dc.subjectRAPD markerspt_PT
dc.subjectISSR markerspt_PT
dc.subjectPlant molecular fingerprintspt_PT
dc.subjectPlant genetic relationshipspt_PT
dc.titleNext-Generation Sequencing (NGS) identified Species-Specific SSR and SNP markers, allow the unequivocal identification of Strawberry Tree (Arbutus unedo L.) germplasm accessions and contribute to assess their genetic relationshipspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMediterranean Institute for Agriculture, Environment and Development
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05183%2F2020/PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1517pt_PT
oaire.citation.titlePlantspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePereira
person.familyNameVera dos Anjos
person.familyNameReis
person.familyNameDias
person.familyNameLeitão
person.givenNameRicardo
person.givenNameIsabela
person.givenNameJoão
person.givenNameAna Carolina
person.givenNameJosé
person.identifier2700941
person.identifier258398
person.identifier.ciencia-id6219-C33A-5BA2
person.identifier.ciencia-idDA17-054E-9131
person.identifier.ciencia-idC818-CA64-011F
person.identifier.ciencia-id2F10-4DE3-123F
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0002-7358-6300
person.identifier.orcid0000-0002-3228-8550
person.identifier.orcid0000-0002-3461-8904
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id57206728570
person.identifier.scopus-author-id7005119796
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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