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Comparison of thiol subproteome of the vent mussel Bathymodiolus azoricus from different Mid-Atlantic Ridge vent sites

2012Journal article Restricted access
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dc.contributor.authorCompany, Rui
dc.contributor.authorTorreblanca, Amparo
dc.contributor.authorCajaraville, Miren
dc.contributor.authorBebianno, Maria JoĂŁo
dc.contributor.authorSheehan, David
dc.date.accessioned2020-04-27T10:45:17Z
dc.date.available2020-04-27T10:45:17Z
dc.date.issued2012
dc.description.abstractDeep-sea hydrothermal mussels Bathymodiolus azoricus live in the mixing zone where hydrothermal fluid mixes with bottom seawater, creating large gradients in the environmental conditions and are one of the most studied hydrothermal species as a model of adaptation to extreme conditions. Thiol proteins, i.e. proteins containing a thiol or sulfhydryl group (SH) play major roles in intracellular stress defense against reactive oxygen species (ROS) and are especially susceptible to oxidation. However, they are not particularly abundant, representing a small percentage of proteins in the total proteome and therefore are difficult to study by proteomic approaches. Activated thiol sepharose (ATS) was used for the rapid and quantitative selection of proteins comprising thiol- or disulfide-containing subproteomes. This study aims to isolate thiol-containing proteins from the gills of B. azoricus collected in distinct hydrothermal vents and to study the thiol-containing subproteome as a function of site-specific susceptibility to ROS. Results show that ATS is a powerful tool to isolate the thiol-containing sub-proteome and differently-expressed protein spots showed significant differences among the three vent sites, supporting previous findings that specific environmental conditions are crucial for ROS formation and that B. azoricus have different susceptibilities to oxidative stress depending on the vent site they inhabit.pt_PT
dc.description.sponsorship5th PCRD Ventox project EVK3CT1999-00003pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/10.1016/j.scitotenv.2012.07.089pt_PT
dc.identifier.issn0048-9697
dc.identifier.urihttp://hdl.handle.net/10400.1/13789
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationA PROTEOMIC APPROACH TO THE INVESTIGATION OF MOLECULAR MECHANISMS INVOLVED IN OXIDATIVE STRESS RESISTANCE IN HYDROTHERMAL AND COASTAL MARINE ORGANISMS
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S004896971201042X?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAdaptationpt_PT
dc.subjectAnimalspt_PT
dc.subjectBivalviapt_PT
dc.subjectGillspt_PT
dc.subjectOxidative stresspt_PT
dc.subjectProteinspt_PT
dc.subjectProteomept_PT
dc.subjectReactive oxygen speciespt_PT
dc.subjectSepharosept_PT
dc.subjectSulfhydryl compoundspt_PT
dc.subjectHydrothermal ventspt_PT
dc.subjectBathymodiolus azoricuspt_PT
dc.subjectThiol sub-proteomept_PT
dc.subjectActivated thiol sepharosept_PT
dc.titleComparison of thiol subproteome of the vent mussel Bathymodiolus azoricus from different Mid-Atlantic Ridge vent sitespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleA PROTEOMIC APPROACH TO THE INVESTIGATION OF MOLECULAR MECHANISMS INVOLVED IN OXIDATIVE STRESS RESISTANCE IN HYDROTHERMAL AND COASTAL MARINE ORGANISMS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F26399%2F2006/PT
oaire.citation.endPage421pt_PT
oaire.citation.startPage413pt_PT
oaire.citation.titleScience of The Total Environmentpt_PT
oaire.citation.volume437pt_PT
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isProjectOfPublication3793dcb2-53f7-464c-bdb8-3fcdd7fc2e6e
relation.isProjectOfPublication.latestForDiscovery3793dcb2-53f7-464c-bdb8-3fcdd7fc2e6e

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