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Typing of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein gene

dc.contributor.authorAmin, H. A.
dc.contributor.authorFonseca, Filomena
dc.contributor.authorSantos, C.
dc.contributor.authorNolasco, Gustavo
dc.date.accessioned2015-06-15T14:13:48Z
dc.date.available2015-06-15T14:13:48Z
dc.date.issued2006
dc.description.abstractThe capsid protein gene of three Egyptian CTV isolates from two locations was amplified by immunocapture RT-PCR and analysed by single stranded conformation polymorphism and sequencing. The CTV isolates studied did not differ significantly in sequence composition and each isolate consisted of very similar haplotypes. Comparison with reference sequences from isolates elsewhere in the world showed that these haplotypes clustered very close to the severe strain T3 from Florida causing quick decline and stem pitting. Analysis of the deduced amino acid sequence showed the epitope characteristic of reactivity with the MCA13 antibody. Sequence comparison with the sequence of an Egyptian isolate (Qaha) available in the Genbank showed a distance of about 8%, suggesting that it had a different origin.
dc.identifier.issn0031-9465
dc.identifier.otherAUT: GNO00324; FFO00315;
dc.identifier.urihttp://hdl.handle.net/10400.1/6250
dc.language.isoeng
dc.peerreviewedyes
dc.relation.isbasedonP-007-HE1
dc.titleTyping of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein gene
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage14
oaire.citation.startPage10
oaire.citation.titlePhytopathologia Mediterranea
oaire.citation.volume45
person.familyNameNolasco
person.givenNameGustavo
person.identifier.orcid0000-0002-7025-6023
person.identifier.scopus-author-id6602625528
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication87a8035f-bc15-44dd-b8c3-4288c5660fff
relation.isAuthorOfPublication.latestForDiscovery87a8035f-bc15-44dd-b8c3-4288c5660fff

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