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Advisor(s)
Abstract(s)
In vitro propagation of Rhododendron ponticum L. subsp. baeticum, an endangered
species present in limited and vulnerable populations as a Tertiary relict in the southern Iberian Peninsula, was attained. Several cytokinin:IAA ratios and a range of zeatin concentrations were evaluated for their effect on shoot multiplication from apical shoots and nodal segments. The type
of cytokinin and the origin of the explant were the most important factors affecting shoot multiplication.
The highest shoot multiplication rate was obtained from single-nodal explants on
medium supplemented with zeatin. Increasing zeatin concentration promotes shoot multiplication independently of explant type, although this effect tends to decrease with higher zeatin concentration.
Shoot growth was higher in apical shoots and it was not stimulated by the presence of
auxin. A number of experiments were conducted to identify suitable procedures for rooting of in vitro produced shoots. The best results in terms of in vitro rooting were obtained with Anderson’s modified medium with macrosalts reduced to one-half, regardless of the auxin or its concentration
in the medium. Although rooting frequency rose to 97% by basal immersion of shoots in auxin concentrated solution followed by in vitro culture on an auxin-free medium, the survival of the plants after 6 months of acclimatization was poor (50%). Best results (100% rooting and survival) were observed for ex vitro rooting. The micropropagated plants from this study were successfully reintroduced into their natural habitat (87% of survival after 8 months).
Description
Keywords
Conservation biotechnology Endangered species Ex vitro rooting Iberian rose bay In vitro culture
Citation
Almeida R., Gonçalves S., Romano A. 2005. In vitro micropropagation of endangered Rhododendron ponticum L. subsp. baeticum (Boisser & Reuter) Handel-Mazzetti, Biodiversity and Conservation 14: 1059-1069.
Publisher
Springer