dc.contributor.author	Vinhas, Maurícia
dc.contributor.author	Araújo, Ana Carolina
dc.contributor.author	Ribeiro, Sónia
dc.contributor.author	Rosário, Luís Brás
dc.contributor.author	Belo, José A.
dc.date.accessioned	2013-05-21T11:25:12Z
dc.date.available	2013-05-21T11:25:12Z
dc.date.issued	2013-05-01
dc.date.updated	2013-05-14T06:33:58Z
dc.description.abstract	Background In the recent years, the use of Doppler-echocardiography has become a standard non-invasive technique in the analysis of cardiac malformations in genetically modified mice. Therefore, normal values have to be established for the most commonly used inbred strains in whose genetic background those mutations are generated. Here we provide reference values for transthoracic echocardiography measurements in juvenile (3 weeks) and adult (8 weeks) 129/Sv mice. Methods Echocardiographic measurements were performed using B-mode, M-mode and Doppler-mode in 15 juvenile (3 weeks) and 15 adult (8 weeks) mice, during isoflurane anesthesia. M-mode measurements variability of left ventricle (LV) was determined. Results Several echocardiographic measurements significantly differ between juvenile and adult mice. Most of these measurements are related with cardiac dimensions. All B-mode measurements were different between juveniles and adults (higher in the adults), except for fractional area change (FAC). Ejection fraction (EF) and fractional shortening (FS), calculated from M-mode parameters, do not differ between juvenile and adult mice. Stroke volume (SV) and cardiac output (CO) were significantly different between juvenile and adult mice. SV was 31.93 ± 8.67 μl in juveniles vs 70.61 ± 24.66 μl in adults, ρ < 0.001. CO was 12.06 ± 4.05 ml/min in juveniles vs 29.71 ± 10.13 ml/min in adults, ρ < 0.001. No difference was found in mitral valve (MV) and tricuspid valve (TV) related parameters between juvenile and adult mice. It was demonstrated that variability of M-mode measurements of LV is minimal. Conclusions This study suggests that differences in cardiac dimensions, as wells as in pulmonary and aorta outflow parameters, were found between juvenile and adult mice. However, mitral and tricuspid inflow parameters seem to be similar between 3 weeks and 8 weeks mice. The reference values established in this study would contribute as a basis to future studies in post-natal cardiovascular development and diagnosing cardiovascular disorders in genetically modified mouse mutant lines.	por
dc.identifier.citation	Cardiovascular Ultrasound. 2013 May 01;11(1):12	por
dc.identifier.doi	10.1186/1476-7120-11-12
dc.identifier.other	AUT:JBE01557;
dc.identifier.uri	http://hdl.handle.net/10400.1/2698
dc.language.iso	eng	por
dc.peerreviewed	yes	por
dc.publisher	BioMed Central	por
dc.rights.uri	http://creativecommons.org/licenses/by/2.0
dc.title	Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice	por
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.issue	12	por
oaire.citation.title	Cardiovascular Ultrasound	por
oaire.citation.volume	11	por
person.familyName	Araújo
person.familyName	Belo
person.givenName	Ana Carolina
person.givenName	José A.
person.identifier.ciencia-id	BF13-08E9-25E6
person.identifier.orcid	0000-0003-2599-1114
person.identifier.orcid	0000-0001-7384-0949
person.identifier.rid	F-4444-2012
person.identifier.scopus-author-id	6602141392
rcaap.rights	openAccess	por
rcaap.type	article	por
relation.isAuthorOfPublication	5fa0558e-3c87-410a-a9d5-88e7816c32cd
relation.isAuthorOfPublication	4bebeb48-6fac-41a9-8363-f849def5e019
relation.isAuthorOfPublication.latestForDiscovery	5fa0558e-3c87-410a-a9d5-88e7816c32cd

Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice

Files

Original bundle

License bundle

Collections