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Advisor(s)
Abstract(s)
Although the biochemical composition and biological properties of the volatile fraction of
the halophyte sea fennel (Crithmum maritimum L.) have been largely described, little is known about
its polar constituents and bioactivities. Here, a hydromethanolic extract of Crithmum maritimum (L.)
leaves was fractionated, and the fractions were evaluated in vitro for antioxidant (using DPPH, ABTS,
and FRAP bioassays), anti-inflammatory (inhibition of NO production in RAW 264.7 macrophages),
antidiabetic (alpha-glucosidase inhibition), neuroprotective (inhibition of acetylcholinesterase),
and skin-protective (tyrosinase and melanogenesis inhibitions) activities. Polar fractions of the
extract were rich in phenolics and, correlatively, displayed a strong antioxidant power. Moreover,
fractions eluted with MeOH20 and MeOH80 exhibited a marked inhibition of alpha-glucosidase
(IC50 = 0.02 and 0.04 mg/mL, respectively), MeOH60 fractions showed a strong capacity to reduce
NO production in macrophages (IC50 = 6.4 µg/mL), and MeOH80 and MeOH100 fractions had
strong anti-tyrosinase activities (630 mgKAE/gDW). NMR analyses revealed the predominance
of chlorogenic acid in MeOH20 fractions, 3,5-dicaffeoylquinic acid in MeOH40 fractions, and
3-O-rutinoside, 3-O-glucoside, 3-O-galactoside, and 3-O-robinobioside derivatives of quercetin
in MeOH60 fractions. These compounds likely account for the strong antidiabetic, antioxidant, and
anti-inflammatory properties of sea-fennel polar extract, respectively. Overall, our results make
sea fennel a valuable source of medicinal or nutraceutical agents to prevent diabetes, inflammation
processes, and oxidative damage.
Description
Keywords
Crithmum maritimum Halophytes Antioxidants Anti-inflammatory agents Anti-ageing activity Antidiabetic activity Bioguiding NMR characterization Phenolic compounds
Citation
Foods 13 (9): 1294 (2024)
Publisher
MDPI