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Effects of water deficit on the activity of nitrate reductase and content of sugars, nitrate and free amino acids in the leaves and roots of sunflower and white lupin plants growing under two nutrient supply regimes

2005Journal article Open access
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dc.contributor.authorCorreia, Maria João
dc.contributor.authorFonseca, Filomena
dc.contributor.authorAzedo-Silva, João
dc.contributor.authorDias, Celeste
dc.contributor.authorDavid, Maria Manuela
dc.contributor.authorBarrote, Isabel
dc.contributor.authorOsório, Maria Leonor
dc.contributor.authorOsório, Júlio
dc.date.accessioned2012-04-13T09:35:42Z
dc.date.available2012-04-13T09:35:42Z
dc.date.issued2005
dc.date.updated2012-04-12T15:11:46Z
dc.description.abstractThe effects of a two weeks soil drying period on the activity of nitrate reductase (NR; EC 1.6.6.6) were studied on Helianthus annuus L. and non-nodulated Lupinus albus L. plants, growing under two nutrient supply regimes. NR activity was assessed in leaf and root extracts, by measuring the activity of the unphosphorylated active form (NRact), the maximal extractable activity (NRmax) and the activation state. To get insight into potential signalling compounds, nitrate, amino acids and soluble sugars concentrations were also quantified. On both species, foliar NRact and NRmax were negatively affected by soil drying and reduced supply of nutrients, the observed changes in NR activity being linearly-correlated with the depletion of nitrate. Similar results were obtained in the roots of sunflower. Conversely, in white lupin roots NRmax was found to be independent of tissue nitrate concentration. Regardless of the species and organ, the activation state of the enzyme was unaffected by the nutrient supply regime. In well-watered sunflower roots only about 50% of the existing NR was unphosphorylated, but the activation state increased significantly in response to drought. In contrast, lupin roots always exhibited NR activation state values close to 80% or even higher. At the leaf level, NR activation state was hardly changed in response to soil drying. The contribution of changes in the concentrations of soluble 5 sugars and amino acids to explain the observed variations in NR activity are discussed.por
dc.identifier.citationCorreia, Maria Joao; Fonseca, Filomena; Azedo-Silva, Joao; Dias, Celeste; David, Maria Manuela; Barrote, Isabel; Osorio, Maria Leonor; Osorio, Julio. Effects of water deficit on the activity of nitrate reductase and content of sugars, nitrate and free amino acids in the leaves and roots of sunflower and white lupin plants growing under two nutrient supply regimes. Physiologia Plantarum, 124, 1, 61-70, 2005.por
dc.identifier.doihttp://dx.doi.org/10.1111/j.1399-3054.2005.00486.x
dc.identifier.issn0031-9317
dc.identifier.otherAUT: JCO00381; FFO00315; JOS01477; MDA00369; IBA00639;
dc.identifier.urihttp://hdl.handle.net/10400.1/1022
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectPhotosynthesispor
dc.subjectSurvivalpor
dc.subjectPhotochemistrypor
dc.subjectPhotoinhibitionpor
dc.titleEffects of water deficit on the activity of nitrate reductase and content of sugars, nitrate and free amino acids in the leaves and roots of sunflower and white lupin plants growing under two nutrient supply regimespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage70por
oaire.citation.issue124(1)por
oaire.citation.startPage61por
oaire.citation.titlePhysiologia Plantarumpor
person.familyNameDavid
person.familyNameBarrote
person.familyNameOsório
person.familyNameOsório
person.givenNameMaria Manuela
person.givenNameIsabel
person.givenNameMaria Leonor
person.givenNameJúlio
person.identifier.ciencia-idE31B-16E0-7562
person.identifier.ciencia-id801C-0AE4-B5CA
person.identifier.orcid0000-0001-6251-7222
person.identifier.orcid0000-0002-0922-0259
person.identifier.orcid0000-0002-7713-6497
person.identifier.orcid0000-0002-4054-4091
person.identifier.ridN-8024-2013
person.identifier.scopus-author-id7402103697
person.identifier.scopus-author-id8659654200
person.identifier.scopus-author-id7006394339
person.identifier.scopus-author-id9938818600
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication8027b4d2-ee8d-4432-be75-7a2342a905a8
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relation.isAuthorOfPublication.latestForDiscovery8027b4d2-ee8d-4432-be75-7a2342a905a8

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