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Genomic variability of prune dwarf virus as affected by agricultural practice

dc.contributor.authorFonseca, Filomena
dc.contributor.authorNeto, J. D.
dc.contributor.authorMartins, V.
dc.contributor.authorNolasco, Gustavo
dc.date.accessioned2015-06-15T14:13:45Z
dc.date.available2015-06-15T14:13:45Z
dc.date.issued2005
dc.description.abstractTwelve new sequences of the coat protein gene of Prune dwarf virus (PDV) variants, obtained from almond trees, are presented. Comparison with previously reported sequences of the same region, obtained from other hosts (plum, cherry and peach) revealed not only the existence of a wider range of variants of PDV than formerly predicted, but also the frequent presence of a mixture of variants in each sample. In spite of the heterogeneity found in almond, the amino acid composition of the domain at the N terminus of the coat protein maintained the potential to form an amphipathic helix, and hence the capacity to serve the previously suggested function of binding the viral RNA during particle formation. Except for synonymous substitutions, measures of nucleotide diversity calculated for the two groups, respectively 13 sequences from almond and 14 sequences from other hosts, were found to be significantly different, with the almond group showing a much higher variability. Analysis of the dendrogram constructed based in all 27 PDV CP sequences did not reveal host specificity, in agreement with previous findings. However, a clear divergence between almond and other hosts sequences could be found. It is discussed that the observed differences between almond and other hosts variants may derive from differences in agricultural practices.
dc.identifier.doihttps://dx.doi.org/10.1007/s00705-005-0507-z
dc.identifier.issn0304-8608
dc.identifier.otherAUT: FFO00315; GNO00324;
dc.identifier.urihttp://hdl.handle.net/10400.1/6226
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relation.isbasedonP-000-203
dc.titleGenomic variability of prune dwarf virus as affected by agricultural practice
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1619
oaire.citation.startPage1607
oaire.citation.titleArchives of Virology
oaire.citation.volume150
person.familyNameNolasco
person.givenNameGustavo
person.identifier.orcid0000-0002-7025-6023
person.identifier.scopus-author-id6602625528
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication87a8035f-bc15-44dd-b8c3-4288c5660fff
relation.isAuthorOfPublication.latestForDiscovery87a8035f-bc15-44dd-b8c3-4288c5660fff

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