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In Proteomics, Differential Analysis is the method of studying 2-D Electrophoresis (2-DE)
images by finding the differences. This method involves comparing the images’ spots in order
to find the missing, unidentified, and/or misplaced proteins. The manual comparison by visual
inspection is a labor-intensive and error-prone task. Matching two gels is not an easy task.
Biologists have to identify and quantify the spots one-by-one.
