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Nitrate reductase activity in green macroalgae as an environmental indicator of temperature and salinity changes and its implication for climate change projections

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dc.contributor.authorMadeira, Hélder T.
dc.contributor.authorAníbal, Jaime
dc.contributor.authorCarvalho, Liliana F.
dc.contributor.authorEsteves, Eduardo
dc.contributor.authorVeiga-Pires, C.
dc.date.accessioned2021-07-28T10:27:13Z
dc.date.available2021-07-28T10:27:13Z
dc.date.issued2020
dc.date.updated2021-07-27T21:20:01Z
dc.description.abstractTemperature and salinity changes can affect nutrient assimilation dynamics in primary producers. Green macroalgae use nitrate as a main source of nitrogen for their metabolism. Nitrate needs to be reduced by nitrate reductase, before amino acids synthesis. Our aims were to study the effect of temperature and salinity changes on nitrate reductase activity (NRA) in Ulva rigida and Enteromorpha clathrata, and to assess if this enzyme can be used as an environmental indicator for changes in such abiotic factors. The study of NRA was carried out using potassium nitrate as substrate and propanol as a membrane permeabilizer, letting the produced nitrite to exit the macroalgae cells into the assay medium, allowing its quantification through a colorimetric method. This procedure was carried out at five temperatures (10, 20, 30, 35 and 40 °C) and three salinities of the assay medium (0, 15 and 36 g/kg). Results show that both Ulva and Enteromorpha have maximum NRA at salinities of 36 g/kg, although nitrate reduction can occur in freshwater or brackish water, but at significantly lower rates. NRA varied significantly with temperature for both macroalgae, although Ulva showed maximum NRA at 30 °C, while NRA peaked at 35 °C in Enteromorpha. Likely adequate models were tentatively fitted to NRAtemperature data at different salinities. NRA is a suitable proxy of the effects of temperature and salinity changes on the ability of green macroalgae to uptake and metabolize nitrogen nutrients and can thus be the base for macroalgae proliferation models under climate change model scenarios.pt_PT
dc.description.sponsorshipGrant.Provided by PTCRIS: 70247pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.authenticusidP-00R-CF2
dc.identifier.doi10.1007/978-3-030-30938-1_2pt_PT
dc.identifier.slugcv-prod-972966
dc.identifier.urihttp://hdl.handle.net/10400.1/16826
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.subjectNitrate reductasept_PT
dc.subjectTemperaturept_PT
dc.subjectSalinitypt_PT
dc.subjectOptimum (Growth) modelpt_PT
dc.titleNitrate reductase activity in green macroalgae as an environmental indicator of temperature and salinity changes and its implication for climate change projectionspt_PT
dc.typebook part
dspace.entity.typePublication
oaire.citation.titleINCREaSE 2019 Proceedings of the 2nd International Congress on Engineering and Sustainability in the XXI Centurypt_PT
person.familyNameAnibal
person.familyNameEsteves
person.familyNameVeiga-Pires
person.givenNameJaime
person.givenNameEduardo
person.givenNameCristina
person.identifier113325
person.identifier.ciencia-idE01D-F785-AB70
person.identifier.ciencia-idC81D-666A-FF24
person.identifier.ciencia-id4A1F-CEDA-E66C
person.identifier.orcid0000-0002-9704-5824
person.identifier.orcid0000-0002-1872-8185
person.identifier.orcid0000-0002-9323-0723
person.identifier.ridA-6080-2010
person.identifier.ridE-9582-2012
person.identifier.ridB-5144-2008
person.identifier.scopus-author-id6506031408
person.identifier.scopus-author-id7003885183
person.identifier.scopus-author-id7801372905
rcaap.cv.cienciaid4A1F-CEDA-E66C | Cristina Veiga Pires
rcaap.rightsrestrictedAccesspt_PT
rcaap.typebookPartpt_PT
relation.isAuthorOfPublication66594510-941f-49ed-86f5-3ef199bc3227
relation.isAuthorOfPublicationaa7fd030-1bcb-4f16-83ad-508346a35f97
relation.isAuthorOfPublicationd1541efa-0eaa-48dc-aea3-a1e64aa0065a
relation.isAuthorOfPublication.latestForDiscoveryaa7fd030-1bcb-4f16-83ad-508346a35f97

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