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Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi

dc.contributor.authorArcher, Margarida
dc.contributor.authorRodrigues, Maria Luisa
dc.contributor.authorAurélio, M.
dc.contributor.authorBiemans, R.
dc.contributor.authorCravador, A.
dc.contributor.authorCarrondo, Maria A.
dc.date.accessioned2012-06-08T10:23:03Z
dc.date.available2012-06-08T10:23:03Z
dc.date.issued2000
dc.date.updated2012-06-07T17:47:28Z
dc.description.abstractCinnamomin (CIN) belongs to a family of 10 kDa proteins designated as elicitins. Some of these proteins induce a hypersensitive response in diverse plant species, leading to resistance against fungal and bacterial plant pathogens. CIN was crystallized by the vapourdiffusion method using either ammonium sulfate or polyethyleneglycol (PEG) as precipitants in solutions buffered at around pH 7. These crystals are isomorphous and belong to the triclinic space group, with unit-cell parameters a = 31.69, b = 36.99, c = 44.09 A Ê , = 76.86, = 84.41, = 80.26 . A frozen crystal diffracted X-rays beyond 1.45 A Ê resolution on a synchrotron-radiation source.por
dc.identifier.citationArcher, Margarida; Rodrigues, Maria Luisa; Aurélio, M.; Biemans, R; Cravador, Alfredo; Carrondo, Maria A.. Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi. Acta Crystallographica , 56, 3, 363-366, 2000.por
dc.identifier.issn0907-4449
dc.identifier.otherAUT: ACR00659;
dc.identifier.urihttp://hdl.handle.net/10400.1/1202
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectBeta-cinnamominpor
dc.subjectElicitinspor
dc.subjectPhytopathogenic fungipor
dc.titleCrystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomipor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage366por
oaire.citation.issue56(3)por
oaire.citation.startPage363por
oaire.citation.titleActa Crystallographicapor
person.familyNameCravador
person.givenNameAlfredo
person.identifier.orcid0000-0002-9831-9815
person.identifier.ridK-7247-2012
person.identifier.scopus-author-id6602537257
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication8c84a9e6-6b7f-4b50-93a2-152c85901722
relation.isAuthorOfPublication.latestForDiscovery8c84a9e6-6b7f-4b50-93a2-152c85901722

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