Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/1310
Título: Actomyosin modulation by peroxynitrite
Autor: Tiago, Teresa
Silva, D.
Santos, A.
Aureliano, M.
Gutiérrez-Merino, Carlos
Palavras-chave: Actomyosin
Data: 2007
Editora: Medimond
Resumo: In the present work we address the oxidative modifications accounting for the structural and functional impairment of the actomyosin complex under the oxidative stress mediated by peroxynitrite (ONOO-). Experiments on purified myosin and actin have shown that submicromolar ONOO- concentrations produce strong inhibition of the F-actin stimulated myosin ATPase activity. The peroxynitrite-induced actomyosin impairment correlated with structural modifications that decrease the thermal stability of both actin and myosin leading to partially unfolded states. The results suggest a major role for the highly reactive cysteines on actin and on myosin and also for some critical methionines on G-actin. 3-nitrotyrosine does not contribute significantly to the observed functional alterations.
Peer review: yes
URI: http://hdl.handle.net/10400.1/1310
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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