Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/3853
Título: Parathyroid hormone-related protein regulates intestinal calcium transport in sea bream (Sparus auratus)
Autor: Fuentes, J.
Figueiredo, J.
Power, Deborah
Canario, Adelino V. M.
Palavras-chave: Parathyroid hormone
Sparus auratus
Data: 1-Nov-2006
Editora: American Physiological Society
Citação: Fuentes, J.; Figueiredo, J.; Power, D. M.; Canario, A. V. M.Parathyroid hormone-related protein regulates intestinal calcium transport in sea bream (Sparus auratus), American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 291, 5, R1499-R1506, 2006.
Resumo: Parathyroid hormone-related protein (PTHrP) is a factor associated with normal development and physiology of the nervous, cardiovascular, immune, reproductive, and musculoskeletal systems in higher vertebrates. It also stimulates whole body calcium uptake in sea bream (Sparus auratus) larvae with an estimated 60% coming from intestinal uptake in seawater. The present study investigated the role of PTHrP in the intestinal calcium transport in the sea bream in vitro. Unidirectional mucosal-to-serosal and serosal-to-mucosal 45Ca fluxes were measured in vitro in duodenum, hindgut, and rectum mounted in Ussing chambers. In symmetric conditions with the same saline, bathing apical and basolateral sides of the preparation addition of piscine PTHrP 1–34 (6 nM) to the serosal surface resulted in an increase in mucosal to serosal calcium fluxes in duodenum and hindgut and a reduction in serosal to mucosal in the rectum, indicating that different mechanisms are responsive to PTHrP along the intestine. In control asymmetric conditions, with serosal normal and mucosal bathed with a saline similar in composition to the intestinal fluid, there was a net increase in calcium uptake in all regions. The addition of 6 nM PTHrP 1–34 increased net calcium uptake two- to threefold in all regions. The stimulatory effect of PTHrP on net intestinal calcium absorption is consistent with a hypercalcemic role for the hormone. The results support the view that PTHrP, alone or in conjunction with recently identified PTH-like peptides, counteracts in vivo the hypocalcemic effects of stanniocalcin.
Descrição: Texto integral disponível através do editor.
Peer review: yes
URI: http://hdl.handle.net/10400.1/3853
DOI: http://dx.doi.org/10.1152/ajpregu.00892.2005
ISSN: 0363-6119
Versão do Editor: http://ajpregu.physiology.org/content/291/5/R1499.full-text.pdf+html
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