Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6750
Título: Development of a highly sensitive bacteria detection assay using fluorescent pH-responsive polymeric micelles
Autor: Mouffouk, Fouzi
Rosa da Costa, Ana
Martins, Jorge
Zourob, Mohammed
Abu-Salah, Khalid
Alrokayan, Salman A.
Palavras-chave: Biosensors
Bacteria detection
Poly(ethylene glycol-b-trimethylsilylmethacrylate)
Self assembled polymers
pH sensitive micelles
Pyrenyl fluorescence
Data: Mar-2011
Editora: Elsevier
Citação: Biosensors and Bioelectronics 26 (2011) 3517–3523
Resumo: The detection and control of bacteria is extremely important in the safety of food products and health systems. The conventional microbiological methods based on culture enrichment techniques and plating procedures are highly sensitive and selective for bacterial detection but are expensive, cumbersome and time-consuming. Here we report the development of a simple and sensitive bioassay to detect Escherichia coli (E. coli) bacteria by using self assembled pH-responsive polymeric micelles that have been bioconjugated to anti-E. coli (capturing agent). Poly(ethylene glycol-b-trimethylsilyl methacrylate), containing silicon moieties that can be cleaved under mildly acidic conditions, was synthesized and selfassembled into micelles, that were loaded with a fluorescent dye (1-methylpyrene). The polymer silicon protecting groups are used as a tool to remotely activate the dye release by means of pH. The high sensitivity of the newly developed bioassay, which is capable of detecting 15 bacteria per milliliter of solution, is due to an amplification effect generated by the optical signal of millions of fluorophores released from a single micelle upon attachment to a bacterium. Fluorescence probing involves the measurements of changes in the emission spectra, through the disappearance of the excimer band, which only occurs when the dye molecules are trapped within the polymeric micelles.
Peer review: yes
URI: http://hdl.handle.net/10400.1/6750
DOI: 10.1016/j.bios.2011.01.037
ISSN: 0956-5663
Versão do Editor: http://ac.els-cdn.com/S0956566311000613/1-s2.0-S0956566311000613-main.pdf?_tid=45c6f7be-532b-11e5-8831-00000aab0f26&acdnat=1441388282_61ed19eb6859b58d76e835494623c5c9
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