Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/7387
Título: Novel triblock co-polymer nanofibre system as an alternative support for embryonic stem cells growth and pluripotency
Autor: Perestrelo, Ana Rubina
Mouffouk, Fouzi
Costa, Ana M. Rosa da
Belo, José
Palavras-chave: Embryonic stem cells
Polymeric nanofibres
Embryonic stem cell culture
Growth support
Gelatin substitute
Data: 2016
Editora: Wiley
Citação: J Tissue Eng Regen Med (in press)
Resumo: Conventionally, embryonic stem cells (ESCs) are cultured on gelatin or over a mitotically inactivated monolayer of mouse embryonic fibroblasts (MEFsi). Considering the lack of versatile, non-animal-derived and inexpensive materials for that purpose, we aimed to find a biomaterial able to support ESC growth in a pluripotent state that avoids the need for laborious and time-consuming MEFsi culture in parallel with mouse ESC (mESC) culture. Undifferentiated mESCs were cultured in a new nanofibre material designed for ESC culture, which is based on the self-assembly of a triblock co-polymer, poly(ethyleneglycol-β-trimethylsilyl methacrylate-β-methacrylic acid), conjugated with the peptide glycine-arginine-glycine-aspartate-serine, to evaluate its potential application in ESC research. The morphology, proliferation, viability, pluripotency and differentiation potential of mESCs were assessed. Compared to conventional stem cell culture methodologies, the nanofibres promoted a higher increase in mESCs number, enhanced pluripotency and were able to support differentiation after long-term culture. This newly developed synthetic system allows the elimination of animal-derived matrices and provides an economic method of ESC culture, made of a complex network of nanofibres in a scale similar to native extracellular matrices, where the functional properties of the cells can be observed and manipulated.
Peer review: yes
URI: http://hdl.handle.net/10400.1/7387
DOI: http://dx.doi.org/ 10.1002/term.1838
ISSN: 1932-6254
Versão do Editor: http://onlinelibrary.wiley.com/doi/10.1002/term.1838/pdf
Aparece nas colecções:DCB2-Artigos (em revistas ou actas indexadas)

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