Browsing by Author "Brito, A. B."
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- Developmental effects of warfin in the skeletogenesis of zebrafish (Danio rerio) larvaePublication . Peres dos Santos, R.; Brito, A. B.; Cancela, Leonor; Gavaia, Paulo J.The vitamin K-dependent gamma carboxylase (VKGC) is responsible for the post-translational modification of glutamate residues to y-carboxy glumatic acid (Gla), in the presence of the co-factor, vitamin K. This modification has important implications, such as physiological homeostasis, signal transduction and bone calcification. This mechanism ensures complete carboxylation of coagulation factors, and matrix proteins like bone Gla protein (bgp) and matrix Gla protein (mgp), being essential for their biological activity.
- Diets affect skeletal development in zebrafish (Danio rerio)Publication . Dionísio, Gisela; Brito, A. B.; Cancela, Leonor; Gavaia, Paulo J.The zebrafish Danio rerio has recently emerged as an eminent vertebrate model for studyign genetics and development. Given the considerable importance of zebrafish as an experimental model, along with the significant economic costs associated with their large-scale use and maintenance of culturing facilities, it is to some extent surprising that their husbandry is poorly developed.
- Expression of osteonectin correlates with levels of fin regeneration in zebrafish (Danio rerioPublication . Brito, A. B.; Cancela, Leonor; Gavaia, Paulo J.Mammals have the ability to regenerate some tissues such as blood and liver, but the majority of organs fail to regenerate. In contrast, zebrafish is capable of regenerating complex organs/tissues such as optic nerve, scales, heart and fins, and is presently one of the most used metazoan in regeneration research. Zebrafish fin is composed of multiple fin rays with bony parts (lepidotrichia) originated by intramembraneous ossification. Fin regeneration is an epimorphic process dependent on formation of a specialized structure (blastema), consisting of mesenchymal-like cells located between stump tissues and the wounded epidermis,
- Expression of osteonectin correlates with levels of fin regeneration in zebrafish (Danio rerio)Publication . Brito, A. B.; Cancela, Leonor; Gavaia, Paulo J.Mammals have the ability to regenerate some tissues such as blood and liver, but the majority of organs fail to regenerate. In contrast, zebrafish is capable of regenerating complex organs/tissues such as optic nerve, scales, heart and fins, and is presently one of the most used metazoan in regeneration research.
- Expression of the oligopeptide transporter PepT1 (Solute carrier family 15, member 1), in fed and starved larval Zebra fish (Danio rerio)Publication . Brito, A. B.; Rønnestad, I.; Gavaia, Paulo J.; S B Viegas, Carla; Cancela, LeonorFish embryos develop while utilizing yolk nutrients supplied by the (...). There normally exists a "first feeding window" between when the larvae (...) able to ingest exogenous feed,and the "point of no return" where (...) exhausted its yolk reserves and also irreversibly degraded critical tissues (...) energetic purposes it feeds is not ingested.
- Gene expression during regeneration of zebrafish (danio rerio) fins: relative expression levels of mineralization – related gla proteinsPublication . Brito, A. B.; Gavaia, Paulo J.; Cancela, LeonorMost animals have the ability to regenerate epidermal injuries yet only a few can regenerate largely severed appendages that comprise several different tissues. Nowadays zebrafish is one of the most used metazoan models in regeneration studies in particular for investigation of molecular events during fin regeneration process. Fin regeneration starts through the formation of a blastema, a set of heterogeneous mesenchyma-like cells located between stump tissues and the wounded epidermis. This event, denominated epimorphic regeneration, comprises strict growth control and cell reprogramming leading to faithful restoration of the lost parts.
- Gene expression during regeneration of zebrafish (danio rerio) fins: relative expression levels of mineralization–related gla proteinsPublication . Brito, A. B.; Gavaia, Paulo J.; Cancela, LeonorMost animals have the ability to regenerate epidermal injuries yet only a few can regenerate largely severed appendages that comprise several different tissues. Nowadays zebrafish is one of the most used metazoan models in regeneration studies in particular for investigation of molecular events during fin regeneration process. Fin regeneration starts through the formation of a blastema, a set of heterogeneous mesenchyma-like cells located between stump tissues and the wounded epidermis. This event, denominated epimorphic regeneration, comprises strict growth control and cell reprogramming leading to faithful restoration of the lost parts.
- Phylogenetic relationships of the North-eastern Atlantic and Mediterranean forms of Atherina (Pisces, Atherinidae)Publication . Francisco, S. M.; Congiu, L.; Stefanni, S.; Castilho, Rita; Brito, A. B.; Ivanova, P. P.; Levy, A.; Cabral, H.; Kilias, G.; Doadrio, I.; Almada, V. C.The genus Atherina (Sand-smelts) is distributed in the Eastern Atlantic Ocean and Mediterranean Sea, extending south along the African coast into the Indian Ocean (Quignard and Pras, 1986). It is a genus of small inshore fishes with many populations living in brackish and freshwater. The taxonomy of the genus has been troublesome due to the intraspecific variability of some of its species, the overlap of characters among many of them and because many nominal species were apparently described based on individuals originating from different localities.
- Phylogeography and demographic history of Atherina presbyter (Pisces: Atherinidae) in the North-eastern Atlantic based on mitochondrial DNAPublication . Francisco, S. M.; Castilho, Rita; Soares, M.; Congiu, L.; Brito, A. B.; Vieira, M. N.; Almada, V. C.A fragment of the mitochondrial control region was used to assess phylogeographic patterns and historical demography of the sand-smelt Atherina presbyter in the North-eastern Atlantic, covering its geographical range. A striking result is the highly marked diVerentiation between the Canary Islands population and western European ones. A genetic structure among European populations of A. presbyter was revealed, with a pattern of isolation-by-distance or a gradient eVect at a scale of hundreds kilometres, an uncommon pattern likely related to the biological and life-history traits of the sand-smelt. The northern European populations present a much lower genetic diversity when compared to southern populations, which is consistent with a recent colonization from southern populations. The results showed signs of Pleistocene signatures, with the population age estimates for the European populations being clearly older than the Last Glacial Maximum (18,000 years bp). Nevertheless, paleotemperature reconstructions show that the sand-smelt could not have inhabited the western European shores during the last glacial phase.
- Regeneration in Zebrafish (Danio rerio) fins: pattern of expression of mineralization–related Gla proteinsPublication . Brito, A. B.; Gavaia, Paulo J.; Cancela, LeonorTeleost fishes have the exceptional ability to largely regenerate severed appendages comprising several different tissues. Fin regeneration starts through the formation of heterogeneous mesenchyma-like cells, named blastema, and located between stump tissues and the wounded epidermis. This event, denominated epimorphic regeneration, comprises strict growth control and cell reprogramming leading to faithful restoration of the lost parts. Matrix Gla Protein (Mgp) and Bone Gla Protein (Bgp, osteocalcin) are small extracellular matrix Gla proteins, members of the vitamin K-dependent (VKD) family.