Browsing by Author "Oliveira, C."
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- Assessment of genetic relationships among Pyrus species and cultivars using AFLP and RAPD markersPublication . Monte-Corvo, L.; Cabrita, L.; Oliveira, C.; Leitão, J. M.Twenty-five Pyrus communis L. cultivars including eight traditional Portuguese pears, and four commercial Pyrus pyrifolia (Burm.) Nak. (Japanese pear or 'nashi') cultivars were analysed by RAPD and AFLP techniques focusing on their molecular discrimination and the assessment of their genetic relatedness. Twenty-five primers generated 324 RAPD markers, among which 271 (84%) were polymorphic. The AFLP technique, using seven primer combinations, revealed a similar level of molecular polymorphisms (87%), representing 418 polymorphic bands among a total of 478 scored in autoradiographs. The high reproducibility of RAPD and AFLP techniques was confirmed comparing DNA samples from different extractions and different digestions of DNA from the same plant. Three genetic similarity matrices and respective dendrograms were elaborated on using RAPD, AFLP or joint RAPD and AFLP data. Both molecular marker techniques proved their reliability to assess genetic relationships among pear cultivars. P. pyrifolia cultivars exhibit a closer genetic relatedness, clustering apart from P. communis cultivars. Within P. communis, 'William's', as well as 'Doyenne du Comice', cluster close to their hybrids. Most of the Portuguese cultivars tend to cluster together, indicating to constitute a relatively independent genetic pool, which can be of interest in pear breeding programs.
- Comparing RAPD and AFLP (TM) analysis in discrimination and estimation of genetic similarities among apple (Malus domestica Borkh.) cultivars - RAPD and AFLP analysis of applesPublication . Goulão, L.; Cabrita, L.; Oliveira, C.; Leitão, J. M.Forty-one apple ( Malus x domestica Borkh.) cultivars were screened for RAPD (Random Amplified Polymorphic DNA) and AFLP (Amplified Fragment Length Polymorphism) markers. RAPD analysis was performed with 35 arbitrary 10-mer primers, selected from 60 primers tested (kits A, C and E, Operon Technologies, Inc.). Of a total of 362 bands observed, 208 (57.5%) were polymorphic. Three-hundred-and-eighty-one AFLP fragments were obtained with 8 primer combinations, of which 218 (57.2%) were polymorphic. Cultivars differentiated through mutation were included in this study and showed identical patterns when analysed with both RAPD and AFLP analysis. The estimated genetic relationships were correlated (r = 73.7%) between the analysis with the two different markers. UPGMA analysis was performed and dendrograms were constructed using either the data apart from each (RAPD and AFLP) method or combined in a single joint matrix. The relationships among the forty-one studied cultivars were basically consistent with the known lineage and geographic origins of the cultivars. The four Portuguese cultivars included in this study clustered together and diverged from the other cultivars. Apparently they constitute an independent genetic pool, which could be of interest for apple plant breeders.
- DOP56 Fibrosis-related transcriptome unveils a distinctive matrix remodelling pattern in penetrating but not in stricturing ileal Crohn's DiseasePublication . Sousa, Helena Tavares; Ferreira. M.; Gullo, I.; Rocha, A. M.; Oliveira, C.; Carneiro, F.; Magro, F.Fibrosis underlies most of CD complications requiring surgery, such as intestinal strictures and penetrating events. We previously demonstrated there were no histopathological differences on transmural fibrosis and fibromuscular changesbetween penetrating and stricturing ileal CD. This study aimed to investigate and compare the fibrosis-related transcriptomic profiles of penetrating and stricturing ileal CD.
- A rota do mosaico romano: o sul da hispânia (Andaluzia e Algarve)Publication . Carrasco, J. M. Campos; Fernández Ugalde, A.; García Dils, S.; Gómez Rodríguez, Á.; Lancha, J.; Oliveira, C.; Rueda Roigé, J. F. de; Vidal Teruel, N. de la o; Bernardes, J. P.A Hispânia meridional corresponde ao sul de duas províncias romanas – a Bética (actual Andaluzia) e a Lusitania (Portugal) – que tiveram grande prosperidade económica, desde a época de Augusto até ao final da Antiguidade (finais do século IV). Os mosaicos que decoravam o chão das casas mais ricas aparecidas nos últimos vinte anos nas escavações em cidades – como em Faro/Ossonoba, na Lusitania e em Écija/ Astigi, na Bética – e nas villae rurais que se encontram maioritariamente nos arredores das cidades – como em Cerro da Vila (Vilamoura)e em Milreu (Estoi), no Algarve – demonstram o elevado nível de desenvolvimento económico,social e cultural das duas províncias durante o período compreendido entre os séculos I e IV d.C.
- Species delimitation in sharpnose sharks (genus Rhizoprionodon) in the western Atlantic Ocean using mitochondrial DNAPublication . Mendonça, F. F.; Oliveira, C.; Burgess, G.; Coelho, Rui; Piercy, A.; Gadig, O. B. F.; Foresti, F.Despite Springer’s (1964) revision of the sharpnose sharks (genus Rhizoprionodon), the taxonomic definition and ranges of Rhizoprionodon in the western Atlantic Ocean remains problematic. In particular, the distinction between Rhizoprionodon terraenovae and R. porosus, and the occurrence of R. terraenovae in South American waters are unresolved issues involving common and ecologically important species in need of fishery management in Caribbean and southwest Atlantic waters. In recent years, molecular markers have been used as efficient tools for the detection of cryptic species and to address controversial taxonomic issues. In this study 415 samples of the genus Rhizoprionodon captured in the western Atlantic Ocean from Florida to southern Brazil were examined for sequences of the COI gene and the D-loop and evaluated for nucleotide differences. The results on nucleotide composition, AMOVA tests, and relationship distances using Bayesian-likelihood method and haplotypes network, corroborates Springer’s (1964) morphometric and meristic finding and provide strong evidence that supports consideration of R. terraenovae and R. porosus as distinct species.