Browsing by Author "Ramsurn, Vimi P."
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- Cloning and characterisation of a fish aldolase B genePublication . Llewellyn, Lynda; Ramsurn, Vimi P.; Sweeney, Glen E.; Wigham, Trevor; Santos, Cecilia; Power, DeborahA full length cDNA clone representing an aldolase mRNA was isolated from a sea bream (Sparus aurutu) liver cDNA library. Sequencing of this clone revealed it to encode a 364 amino acid protein with 74% amino acid identity to human aldolase B and slightly lower similarity to human aldolase A and C. In view of the sequence data and of Northern blot analysis showing strong expression of a 1.6 kb transcript in liver it was concluded that the cloned gene represents aldolase B. This clone represents the first aldolase gene to be sequenced from any fish species thus providing new data on the evolution of the vertebrate aldolase gene family.
- Cloning and expression of an elongation factor-1α in sea bream ( Sparus aurata ) larvae and adult tissuePublication . Nowell, M. A.; Power, Deborah; Guerreiro, P. M.; Llewellyn, Lynda; Ramsurn, Vimi P.; Wigham, Trevor; Sweeney, Glen E.A clone encoding the polypeptide elongation factor EF-1a was isolated from a complementary DNA library prepared from sea bream (Spartus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1a in other animal species. EF-1a messenger RNA is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. This increase presumably reflects midblastula transition in this species. In adult sea bream, EF-1a appeared to have a relatively uniform distribution across all the tissues analyzed.
- Cloning and sequencing of a full-length sea bream (Sparus aurata) beta-actin cDNAPublication . Santos, Cecilia; Power, Deborah; Kille, Peter; Llewellyn, Lynda; Ramsurn, Vimi P.; Wigham, Trevor; Sweeney, Glen E.A full-length cDNA clone encoding beta-actin (b-actin) was isolated from a sea bream (Sparus aurata) liver cDNA library. Sequencing of this clone reveals an open reading frame encoding a 375 amino acid protein that shares a high degree of conservation to other known actins. The sea breamb-actin sequence showed 98% identity to carp and human b-actin and 95% and 94% identity to sea squirt and Dictyostelium cytoplasmic actins, respectively.
- Cloning, characterisation and expression of the apolipoprotein A-I gene in the sea bream (Sparus aurata)Publication . Llewellyn, Lynda; Ramsurn, Vimi P.; Wigham, Trevor; Sweeney, Glen E.; Power, DeborahA full length cDNA clone representing apolipoprotein A-I was isolated from a sea bream (Sparus aurata) liver library. The clone encodes a 261 amino acid protein which shows highest amino acid identity (38%) with salmon apolipoprotein A-I. Northern blot analysis showed strong expression of a 1.4 kb transcript in liver with lower expression in intestine. Expression of apolipoprotein A-I in intestine was markedly reduced by treatment with triiodothyronine (T3). ß 1998 Elsevier Science B.V. All rights reserved.
- Expression of thyroid hormone receptor during early development of the sea bream (Sparus aurata)Publication . Llewellyn, Lynda; Ramsurn, Vimi P.; Sweeney, Glen E.; Wigham, Trevor; Power, DeborahThe thyroid hormones thyroxine (T4) and triiodothyronine (T3) are crucial to many aspects of vertebrate growth, development, and metabolism. They act through intracellular receptor proteins which act directly on target genes. Although the role of thyroid hormones in fish, especially in early development, is not well understood, thyroid hormones are passed to eggs by broodfish during spawning and are implicated in fish development.1,2 Sea bream (Sparus aurata) aquaculture has grown rapidly in importance in the European Community, particularly in southern Portugal. However, its further development is hindered by the high larval mortality rate and incidence of malformations. This work investigates the significance of thyroid hormones in sea bream development by cloning the thyroid hormone receptor (TR) and analyzing its expression during larval development.