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- Relationship between postharvest diseases resistance and mineral composition of citrus fruitPublication . Nunes, C.; Duarte, Amílcar; Manso, T.; Weiland, C.; Garcia, J. M.; Cayuela, J. A.; Yousfi, K.; Martínez, M.C.; Salazar, Miguelreen and blue moulds, due to the pathogenic action of Penicillium digitatum and Penicillium italicum respectively are the main cause of orange losses during postharvest. Under Mediterranean climate conditions, both together are responsible for 80% of total postharvest citrus fruit decay. The type of orchard production system, field location with different types of climate and soil has a main influence on mineral composition of fruits. The mineral composition of fruits can have a significant impact on fruit quality and shelf life during postharvest period. These include effects on fruit colour, texture, disease susceptibility, juice composition and development of physiological disorders. Oranges from different regions from South of Spain and Portugal and from three different production systems (conventional, integrated and organic) were studied to evaluate whether both factors (origin and production system) affected the degree of fruit sensitivity to decay. Results indicate that the sensitivity to green or blue mould is determined better by the origin of fruit than by the system of production.
- Efficacy of electrolyzed water to inactivate foodborne pathogens on fresh-cut applesPublication . Graça, Ana; Nunes, C.; Quintas, Célia; Abadias, Maribel; Usall, J.; Salazar, MiguelChlorine is the most common disinfectant used in the fresh-cut industry but nowadays environmental and health risks have led to the need to find new sanitizers. Electrolyzed water (EW) appears to be a promising alternative. In this work, disinfection efficacy of acidic (AEW) and neutral (NEW) electrolyzed water in fresh-cut apple slices inoculated with Escherichia coli, Listeria innocua or Salmonella choleraesuis was studied. Apple slices were inoculated with a 10(7) cfu/ml suspension of pathogens and treated with the sanitizer EW solutions, with 100 or 50 ppm of free chlorine solutions and with distilled water. Population reduction was determined 30 min after washings and untreated apple slices were used as control in all assays. AEW100 was the treatment with more effective bactericidal activity followed by NEW100 and AEW50. EW had higher or similar efficacy than chlorine treatments in all tested conditions.
- Low dose UV-C illumination as an eco-innovative disinfection system on minimally processed applesPublication . Graça, Ana; Salazar, Miguel; Quintas, Célia; Nunes, C.In this study, the efficacy of UV-C illumination for inactivate Escherichia coli, Listeria innocua or Salmonella enterica, individually or in a mixture, in vitro and on apple slices was determined. Apple slices inoculated with a 10(7) cfu/mL suspension of above indicated pathogens were irradiated on both sides with UV-C illumination, with doses of 0.5 and 1.0 kJ/m(2). UV-C illumination disinfection efficacy was compared to that of washings with sodium hypochlorite at 100 ppm of free chlorine and with distilled water. Bactericidal activity of each treatment was assessed after 30 min and after 7 and 15 days of storage at 4 degrees C. Results showed that UV-C illumination at 1.0 kJ/m(2) could be an alternative to the wash with hypochlorite solutions. On the in vitro study, these doses completely inhibited the growth of the three bacteria either as pure cultures or in a mixture. In fresh-cut apple, the pathogens were also affected by the UV-C illumination, the 1.0 kJ/m(2) dosage being the one that resulted in higher bacteria inhibition in almost every case. The UV-C treatment did not affect the quality properties of fresh-cut apple. 2013 Elsevier B.V. All rights reserved.
- Influencia del modo de producción en la sensibilidad poscosecha de cítricos a PenicilliumPublication . Nunes, C.; García, J. M.; Cayuela, J. A.; Manso, T.; Yousfi, K.; Salazar, Miguel; Duarte, AmílcarUtilizando naranjas (citrus sinensil L. cv. "Valencia") de cuatro diferentes procedencias de España (Lepe y Rio Tinto en Huelva) y Portugal (Tavira y Silves) y tres diferentes modos de producción (convencional, integrada y ecológico) se ha estudiado si ambos factores (procedencia y sistema de producción) afectaban sobre grado de sensibilidad a Penicillium digitatun y P. italicum de estos frutos, evaluando la incidencia de podrido que presentaban los mismos tras una conservación de 7 días a 24 º c, después de ser inoculdos con los citados hongos.