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Establishment of an induced pluripotent cell line (ABCRIi001-A) from an elderly female for ageing research

2024-12Artigo científico Acesso aberto
http://hdl.handle.net/10400.1/26482
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Human induced pluripotent stem cells (hiPSCs) hold promises to model and understand human diseases, including those associated with ageing. Here, we describe ABCRIi001-A, a hiPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 79-year-old female enrolled in a study for development of an ageing score (ALFA Score). PBMCs were reprogrammed using three Sendai virus-based reprogramming vectors (hKOS, hc-Myc, and hKlf4). ABCRIi001-A showed normal morphology and karyotype, viral clearance, absence of genomic aberrations, and their pluripotency was confirmed by expression of pluripotency-related markers and their ability to differentiate into the three germ layers. ABCRIi001-A is valuable for ageing-related studies.

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http://hdl.handle.net/10400.1/26482

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Elsevier

DOI

10.1016/j.scr.2024.103576

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FCB2-Artigos (em revistas ou actas indexadas)
ABC2-Artigos (em revistas ou actas indexadas)
FCT2-Artigos (em revistas ou actas indexadas)

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