Publication
Decavanadate and metformin-decavanadate effects in human melanoma cells
| dc.contributor.author | de Sousa-Coelho, Ana Luísa | |
| dc.contributor.author | Aureliano, Manuel | |
| dc.contributor.author | Fraqueza, Gil | |
| dc.contributor.author | Serrão, Gisela | |
| dc.contributor.author | Gonçalves, João | |
| dc.contributor.author | Sánchez-Lombardo, Irma | |
| dc.contributor.author | Link, Wolfgang | |
| dc.contributor.author | Ferreira, Bibiana | |
| dc.date.accessioned | 2022-12-07T11:24:33Z | |
| dc.date.available | 2022-12-07T11:24:33Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Decavanadate is a polyoxometalate (POMs) that has shown extensive biological activities, including antidiabetic and anticancer activity. Importantly, vanadium-based compounds as well as antidiabetic biguanide drugs, such as metformin, have shown to exert therapeutic effects in melanoma. A combination of these agents, the metformin-decavanadate complex, was also recognized for its antidiabetic effects and recently described as a better treatment than the monotherapy with metformin enabling lower dosage in rodent models of diabetes. Herein, we compare the effects of decavanadate and metformin-decavanadate on Ca2+-ATPase activity in sarcoplasmic reticulum vesicles from rabbit skeletal muscles and on cell signaling events and viability in human melanoma cells. We show that unlike the decavanadate-mediated non-competitive mechanism, metformin-decavanadate inhibits Ca2+-ATPase by a mixed-type competitive-non-competitive inhibition with an IC50 value about 6 times higher (87 mu M) than the previously described for decavanadate (15 mu M). We also found that both decavanadate and metformin-decavanadate exert antiproliferative effects on melanoma cells at 10 times lower concentrations than monomeric vanadate. Western blot analysis revealed that both, decavanadate and metformin-decavanadate increased phosphorylation of extracellular signal-regulated kinase (ERK) and serine/ threonine protein kinase AKT signaling proteins upon 24 h drug exposure, suggesting that the anti-proliferative activities of these compounds act independent of growth-factor signaling pathways. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1016/j.jinorgbio.2022.111915 | pt_PT |
| dc.identifier.issn | 0162-0134 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/18596 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Decavanadate | pt_PT |
| dc.subject | Metformin | pt_PT |
| dc.subject | Metformin-decavanadate | pt_PT |
| dc.subject | Polyoxometalates | pt_PT |
| dc.subject | Polyoxovanadates | pt_PT |
| dc.subject | Skin cancerMelanoma | pt_PT |
| dc.subject | Cell signaling | pt_PT |
| dc.title | Decavanadate and metformin-decavanadate effects in human melanoma cells | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMED-ONC%2F4167%2F2020/PT | |
| oaire.citation.startPage | 111915 | pt_PT |
| oaire.citation.title | Journal of Inorganic Biochemistry | pt_PT |
| oaire.citation.volume | 235 | pt_PT |
| oaire.fundingStream | 3599-PPCDT | |
| person.familyName | De Sousa-Coelho | |
| person.familyName | Aureliano | |
| person.familyName | Fraqueza | |
| person.familyName | Serrão Fernandes | |
| person.familyName | ferreira | |
| person.givenName | Ana Luísa | |
| person.givenName | Manuel | |
| person.givenName | Gil | |
| person.givenName | Lina Gisela | |
| person.givenName | Bibiana | |
| person.identifier | 584146 | |
| person.identifier | 1179689 | |
| person.identifier.ciencia-id | 691B-A574-28ED | |
| person.identifier.ciencia-id | AA14-3490-DC5E | |
| person.identifier.ciencia-id | 1317-C7C9-5BDA | |
| person.identifier.ciencia-id | 9517-5590-381E | |
| person.identifier.ciencia-id | A311-E925-09C5 | |
| person.identifier.orcid | 0000-0002-8451-4302 | |
| person.identifier.orcid | 0000-0003-4858-3201 | |
| person.identifier.orcid | 0000-0003-2969-9292 | |
| person.identifier.orcid | 0000-0001-9952-2228 | |
| person.identifier.orcid | 0000-0003-4772-9395 | |
| person.identifier.rid | I-3283-2012 | |
| person.identifier.rid | A-3552-2013 | |
| person.identifier.scopus-author-id | 6603412860 | |
| person.identifier.scopus-author-id | 54683851800 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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