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Publication
Proteomic approach to skin regeneration in a marine teleost. Modulation by oestradiol-17β
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Advisor(s)
Abstract(s)
Skin and scale formation and regeneration in
teleosts have mainly been described from a morphological
perspective, and few studies of the underlying molecular
events exist. The present study evaluates (1) the change in
the skin proteome during its regeneration in a marine teleost
fish (gilthead sea bream, Sparus aurata) and (2) the impact of
oestradiol-17β (Ε2) on regeneration and the involvement of
oestrogen receptor (ER) isoforms. Thirty-five candidate proteins
were differentially expressed (p<0.05) between intact and
regenerated skin proteome 5 days after scale removal, and 27
proteins were differentially expressed after E2 treatment.
Agglomerative hierarchical clustering of the skin proteome
revealed that the skin treated with E2 clustered most closely
to intact skin, while regenerating untreated skin formed an
independent cluster. Gene Ontology classification associated
the differentially expressed proteins in E2-treated skin with
developmental processes and cellular morphogenesis. The proteins
modified during skin regeneration suggest a balance
exists between immune response and anatomical repair.
Overall, the results indicate that, even after 5 days regeneration, the composition of mature skin is not attained, and endocrine
factors, in particular E2, can accelerate wound repair acting
possibly via ERβs expressed in the skin-scales. Several
candidate proteins probably involved in scale development,
osteoglycin, lipocalin2 and lamin A and the transcription
factors PHD and grainyhead were identified. Future studies
of fish skin regeneration will be required to provide further
insight into this multistage process, and the present study
indicates it will be useful to explore immune adaptations of
epithelia permanently exposed to an aqueous environment.
Description
Keywords
Gilthead sea bream Ostrogen receptors Oesteoglycin Scale development Wound healing
Citation
Antoni Ibarz & Patricia I. S. Pinto & Deborah M. Power, "Proteomic approach to skin regeneration in a marine teleost. Modulation by oestradiol-17β" in Mar Biotechnol (2013) 15:629–646.
Publisher
Springer