Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/2924
Título: Study of modes of action of the biocontrol agent metschnikowia and auensis pbc-2
Autor: Manso, Teresa
Vero, Silvana
González, M. Belén
Nunes, Carla
Palavras-chave: Biocontrol agent
Inhibitory compounds
Iron competition
Lytic enzymes
Mode of action
Data: 2010
Editora: Universidade do Algarve
Resumo: Metschnikowia andauensis NCYC 3728 (PBC-2) is an effective antagonist against the postharvest pathogens Penicillium expansum, Botrytis cinerea and Rhizopus stolofiner on pome fruits, however its mode of action is unknown. The ability of this strain to produce inhibitory compounds in 4 distinct media (PDA, NYDA, YPDA, CJA) at 3 temperatures (1, 25, 30 ºC), was investigated. It was also assayed the competition for iron in media with different iron concentrations and characterized the capability of PBC-2 produce and secrete fungal cell wall lytic enzymes, like chitinase, protease, and glucanase in a culture media with fungal pathogen cell wall as unique carbon source. M. andauensis PBC-2 did not show any inhibition zone to cope pathogens in any of the tested media. The results obtained in this study suggest that the production and secretion of lytic enzymes is not the main or more important mode of action of the new biocontrol agent PBC-2, since the production of chitinase was observed only past 5 and 7 d of incubation, and the production of -1.3-glucanases and proteases was not observed, which mean that the biocontrol agent PBC-2 have more than one mechanism of action.
Descrição: Proceedings of the International Conference “Environmentally friendly and safe technologies for quality of fruit and vegetables”, held in Universidade do Algarve, Faro, Portugal, on January 14-16, 2009. This Conference was a join activity with COST Action 924.
Peer review: yes
URI: http://hdl.handle.net/10400.1/2924
Aparece nas colecções:UED01-Edições UAlg

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