Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/5397
Título: Dietary keto-acid feed-back on pituitary activity in gilthead sea bream: effects of oral doses of AKG. A proteomic approach
Autor: Ibarz, Antoni
Costa, Rita
Harrison, Adrian P.
Power, Deborah
Palavras-chave: Neuroendocrine
Pituitary proteome
Data: 2010
Editora: Elsevier
Citação: Antoni Ibarz, Rita Costa, Adrian P. Harrison, Deborah M. Power, "Dietary keto-acid feed-back on pituitary activity in gilthead sea bream: Effects of oral doses of AKG. A proteomic approach" in General and Comparative Endocrinology 169 (2010) 284–292.
Resumo: The influence of a daily oral dose of alpha-ketoglutarate (AKG, 0.1 g/kg body weight), an intermediate metabolite in the Krebs cycle and a dietary additive, on the pituitary proteome of gilthead sea bream was determined by two-dimensional electrophoresis (2-DE). A high-resolution map of the sea bream pituitary proteome was generated. Proteins with a modified expression between Controls and AKG treated fish were further analysed by MALDI-TOF/TOF-MS and liquid chromatography combined with a nanoelectrospray (LC–MS/MS). The main changes in the proteome induced by AKG treatment were grouped. Metabolic proteins up-regulated with AKG supplementation included fructose-bis-phosphate aldolase, glyceraldehyde-phosphate dehydrogenase and malate dehydrogenase, all related to glucose metabolism (p < 0.000). Protein folding related up-regulation with AKG supplementation included two isoforms of heat shock proteins as well as cyclophylin and chaperonin (p < 0.000). An unexpected form of apolipoprotein- A-1 with lower molecular weight (15–16 kDa) was evidenced as being highly abundant in the pituitary proteome of Controls, yet it was down-regulated by AKG treatment. Finally, proteins found to be associated with regeneration of neural function namely cofilin and Vat-protein were up-regulated after AKG supplementation. The only hormone to be modified by AKG treatment was somatolactin, which was significantly down-regulated cf. Controls. In summary, these results provide evidence of a potential endocrine/metabolic regulatory loop activated by AKG supplementation.
Peer review: yes
URI: http://hdl.handle.net/10400.1/5397
DOI: http://dx.doi.org/10.1016/j.ygcen.2010.09.010
doi: 10.1016/j.ygcen.2010.09.010
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