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Advisor(s)
Abstract(s)
The influence of a daily oral dose of alpha-ketoglutarate (AKG, 0.1 g/kg body weight), an intermediate
metabolite in the Krebs cycle and a dietary additive, on the pituitary proteome of gilthead sea bream
was determined by two-dimensional electrophoresis (2-DE). A high-resolution map of the sea bream
pituitary proteome was generated. Proteins with a modified expression between Controls and AKG treated
fish were further analysed by MALDI-TOF/TOF-MS and liquid chromatography combined with a nanoelectrospray
(LC–MS/MS). The main changes in the proteome induced by AKG treatment were grouped.
Metabolic proteins up-regulated with AKG supplementation included fructose-bis-phosphate aldolase,
glyceraldehyde-phosphate dehydrogenase and malate dehydrogenase, all related to glucose metabolism
(p < 0.000). Protein folding related up-regulation with AKG supplementation included two isoforms of
heat shock proteins as well as cyclophylin and chaperonin (p < 0.000). An unexpected form of apolipoprotein-
A-1 with lower molecular weight (15–16 kDa) was evidenced as being highly abundant in the pituitary
proteome of Controls, yet it was down-regulated by AKG treatment. Finally, proteins found to be
associated with regeneration of neural function namely cofilin and Vat-protein were up-regulated after
AKG supplementation. The only hormone to be modified by AKG treatment was somatolactin, which
was significantly down-regulated cf. Controls. In summary, these results provide evidence of a potential
endocrine/metabolic regulatory loop activated by AKG supplementation.
Description
Keywords
Neuroendocrine Somatolactin Pituitary proteome
Citation
Antoni Ibarz, Rita Costa, Adrian P. Harrison, Deborah M. Power, "Dietary keto-acid feed-back on pituitary activity in gilthead sea bream: Effects of oral doses of AKG. A proteomic approach" in General and Comparative Endocrinology 169 (2010) 284–292.
Publisher
Elsevier