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Advisor(s)
Abstract(s)
In the sea bream Sparus auratus three stage-specific fast troponin T (fTnT) isoforms have been cloned and correspond to embryonic-, larval- and adult-specific
isoforms. Characterisation, using database searches, of the putative genomic organisation of Fugu rubripes and Tetraodon nigroviridis fTnT indicates that alternative exon splicing in the 59 region of the gene generates the different isoforms. Moreover, comparison of teleost fTnTs suggests
that alternative splicing of fTnT appears to be common in teleosts. A different temporal expression pattern for each fTnT splice variant is found during sea bream development
and probably relates to differing functional demands, as a highly acidic embryonic form (pI 5.16) is substituted by a basic larval form (pI 9.57). Thyroid hormones (THs), which
play an important regulatory role in muscle development in flatfish and tetrapods, appear also to influence TnT gene expression in the sea bream. However, THs have a
divergent action on different sea bream TnT genes and although the slow isoform (sTnT1) is TH-responsive, fTnT, sTnT2 and the itronless isoform (iTnT) are unaffected. The
present results taken together with those published for flatfish seem to suggest differences may exist in the regulation of larval muscle development in teleosts.
Description
Keywords
Fast muscle Slow muscle Splice variants Teleost Temporal gene expression profile Thyroid hormones
Citation
M. A. Campinho, G. E. Sweeney and D. M. Power, "Regulation of troponin T expression during muscle development in sea bream Sparus auratus Linnaeus. The potential role of thyroid hormones" in The Journal of Experimental Biology 209, 4751-4767.
Publisher
Company of Biologists