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Publications

Gene expression during regeneration of zebrafish (danio rerio) fins: relative expression levels of mineralization–related gla proteins

Publication . Brito, A. B.; Gavaia, Paulo J.; Cancela, Leonor

Most animals have the ability to regenerate epidermal injuries yet only a few can regenerate largely severed appendages that comprise several different tissues. Nowadays zebrafish is one of the most used metazoan models in regeneration studies in particular for investigation of molecular events during fin regeneration process. Fin regeneration starts through the formation of a blastema, a set of heterogeneous mesenchyma-like cells located between stump tissues and the wounded epidermis. This event, denominated epimorphic regeneration, comprises strict growth control and cell reprogramming leading to faithful restoration of the lost parts.

2007Conference object

Unknown

Effect of warfarin in zebrafish (Danio rerio) bone formation during caudal fin regeneration

Publication . Dionísio, Gisela; Bensimon-Brito, A.; Gavaia, Paulo J.; Cancela, Leonor

In the last decade, fish has emerged as an important organism for studies on skeletal development in vertebrates, and evidence has been accumulated showing that zebrafish is a suitable system to perform phenotype-based drug screens. The ability to regenerate epidermal injuries is a general feature of most organisms yet only a few can fully regenerate severed appendages comprising several different tissues. Zebrafish is one of the most used models for regeneration studies, creating a powerful tool to study de novo bone formation without affecting vital development processes.

2011Conference object

Unknown

Regeneration in Zebrafish (Danio rerio) fins: pattern of expression of mineralization–related Gla proteins

Publication . Brito, A. B.; Gavaia, Paulo J.; Cancela, Leonor

Teleost fishes have the exceptional ability to largely regenerate severed appendages comprising several different tissues. Fin regeneration starts through the formation of heterogeneous mesenchyma-like cells, named blastema, and located between stump tissues and the wounded epidermis. This event, denominated epimorphic regeneration, comprises strict growth control and cell reprogramming leading to faithful restoration of the lost parts. Matrix Gla Protein (Mgp) and Bone Gla Protein (Bgp, osteocalcin) are small extracellular matrix Gla proteins, members of the vitamin K-dependent (VKD) family.

2006Conference object

Open Access

Expression of osteonectin correlates with levels of fin regeneration in zebrafish (Danio rerio

Publication . Brito, A. B.; Cancela, Leonor; Gavaia, Paulo J.

Mammals have the ability to regenerate some tissues such as blood and liver, but the majority of organs fail to regenerate. In contrast, zebrafish is capable of regenerating complex organs/tissues such as optic nerve, scales, heart and fins, and is presently one of the most used metazoan in regeneration research. Zebrafish fin is composed of multiple fin rays with bony parts (lepidotrichia) originated by intramembraneous ossification. Fin regeneration is an epimorphic process dependent on formation of a specialized structure (blastema), consisting of mesenchymal-like cells located between stump tissues and the wounded epidermis,

2007Conference object

Unknown

Warfarin effects in the skeletal development of zebrafish (Danio rerio)

Publication . Peres dos Santos, R.; Bensimon-Brito, A.; Gavaia, Paulo J.; Cancela, Leonor

Vitamin K-dependent gamma carboxylation (VKGC) is crucial for posttranslational modification of glutamate residues to form α-carboxy glutamic acid (Gla) in the presence of reduced vitamin K, molecular oxygen, and carbon dioxide (1). This modification has important implications, mainly physiological, like homeostasis, signal transduction and bone calcification. This mechanism ensures complete carboxylation of coagulation factors, and proteins like bone Gla protein (BGP) and matrix Gla protein (MGP), being essential for their biological activity. Warfarin is a known anticoagulant that inhibits the action of VKGC (2), inhibiting consequently the activity of the referred proteins.

2009Conference object

Open Access