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Publication
Type I Diabetes in Zebrafish reduces sperm quality and increases Insulin and Glucose transporter transcripts
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Advisor(s)
Abstract(s)
Type I diabetes is a prominent human pathology with increasing incidence in the population;
however, its cause is still unknown. This disease promotes detrimental effects on reproduction, such
as lower sperm motility and DNA integrity. Hence, the investigation of the underlying mechanisms
of this metabolic disturbance in reproduction and its transgenerational consequences is of the utmost
importance. The zebrafish is a useful model for this research considering its high homology with
human genes as well as its fast generation and regeneration abilities. Therefore, we aimed to
investigate sperm quality and genes relevant to diabetes in the spermatozoa of Tg(ins:nfsb-mCherry)
zebrafish, a model for type I diabetes. Diabetic Tg(ins:nfsb-mCherry) males showed significantly
higher expression of transcripts for insulin a (insa) and glucose transporter (slc2a2) compared to controls.
Sperm obtained from the same treatment group showed significantly lower sperm motility, plasma
membrane viability, and DNA integrity compared to that from the control group. Upon sperm
cryopreservation, sperm freezability was reduced, which could be a consequence of poor initial
sperm quality. Altogether, the data showed similar detrimental effects related to type I diabetes
in zebrafish spermatozoa at the cellular and molecular levels. Therefore, our study validates the
zebrafish model for type I diabetes research in germ cells.
Description
Keywords
Zebrafish Type I diabetes Sperm motility DNA integrity
Citation
International Journal of Molecular Sciences 24 (8): 7035 (2023)
