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Type I Diabetes in Zebrafish reduces sperm quality and increases Insulin and Glucose transporter transcripts

2023-04-11Journal article Open access
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dc.contributor.authorDiogo, Patricia
dc.contributor.authorMartins, Gil
dc.contributor.authorF. Simao, Marcio
dc.contributor.authorMarreiros, Ana
dc.contributor.authorEufrásio, Ana Catarina
dc.contributor.authorCabrita, Elsa
dc.contributor.authorJ. Gavaia, Paulo
dc.date.accessioned2023-05-03T09:30:50Z
dc.date.available2023-05-03T09:30:50Z
dc.date.issued2023-04-11
dc.date.updated2023-04-27T13:51:30Z
dc.description.abstractType I diabetes is a prominent human pathology with increasing incidence in the population; however, its cause is still unknown. This disease promotes detrimental effects on reproduction, such as lower sperm motility and DNA integrity. Hence, the investigation of the underlying mechanisms of this metabolic disturbance in reproduction and its transgenerational consequences is of the utmost importance. The zebrafish is a useful model for this research considering its high homology with human genes as well as its fast generation and regeneration abilities. Therefore, we aimed to investigate sperm quality and genes relevant to diabetes in the spermatozoa of Tg(ins:nfsb-mCherry) zebrafish, a model for type I diabetes. Diabetic Tg(ins:nfsb-mCherry) males showed significantly higher expression of transcripts for insulin a (insa) and glucose transporter (slc2a2) compared to controls. Sperm obtained from the same treatment group showed significantly lower sperm motility, plasma membrane viability, and DNA integrity compared to that from the control group. Upon sperm cryopreservation, sperm freezability was reduced, which could be a consequence of poor initial sperm quality. Altogether, the data showed similar detrimental effects related to type I diabetes in zebrafish spermatozoa at the cellular and molecular levels. Therefore, our study validates the zebrafish model for type I diabetes research in germ cells.pt_PT
dc.description.sponsorshipLA/P/0101/2020; ALG-01-0145-FEDER-022121; ZEBRABLOOM-ALG-01-0247-FEDER-039896pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInternational Journal of Molecular Sciences 24 (8): 7035 (2023)pt_PT
dc.identifier.doi10.3390/ijms24087035pt_PT
dc.identifier.eissn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.1/19512
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationThe influence of inflammatory processes in fish skeletal development: the crosstalk between skeletal and immune systems
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectZebrafishpt_PT
dc.subjectType I diabetespt_PT
dc.subjectSperm motilitypt_PT
dc.subjectDNA integritypt_PT
dc.titleType I Diabetes in Zebrafish reduces sperm quality and increases Insulin and Glucose transporter transcriptspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleThe influence of inflammatory processes in fish skeletal development: the crosstalk between skeletal and immune systems
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F146378%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.citation.issue8pt_PT
oaire.citation.startPage7035pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume24pt_PT
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDiogo
person.familyNameMartins
person.familyNameSimão
person.familyNameMarreiros
person.familyNameCabrita
person.familyNameGavaia
person.givenNamePatricia
person.givenNameGil
person.givenNameMárcio
person.givenNameAna
person.givenNameElsa
person.givenNamePaulo
person.identifier.ciencia-idFD18-ED81-FFB5
person.identifier.ciencia-idD11F-8D8A-8EA1
person.identifier.ciencia-idF819-2D45-A17F
person.identifier.ciencia-id9A12-9450-7051
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0002-0804-5014
person.identifier.orcid0000-0002-1344-2954
person.identifier.orcid0000-0001-9658-0895
person.identifier.orcid0000-0001-9410-4772
person.identifier.orcid0000-0003-3864-8841
person.identifier.orcid0000-0002-9582-1957
person.identifier.ridE-8523-2012
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id36695981300
person.identifier.scopus-author-id35739158900
person.identifier.scopus-author-id57194785077
person.identifier.scopus-author-id26645697000
person.identifier.scopus-author-id6507104377
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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