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Immunohistochemical detection of estrogen receptors in fish scales
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Orientador(es)
Resumo(s)
Calcium mobilization from internal stores, such as scales, induced by 17b-estradiol during sexual maturation in salmonids is well documented. This calcium mobilization from scales is proposed to be mediated by the estrogen receptor (ER). However, the ER subtypes involved and signaling mechanisms
responsible for this effect remain to be fully characterized. In the present study, we have localized ERa, ERba and ERbb proteins in juvenile and adult sea bream (Sparus auratus) and Mozambique tilapia
(Oreochromis mossambicus) scales by immunohistochemistry with sea bream ER subtype specific antibodies.
The three ERs were detected in isolated or small groups of round cells, in the basal layer of the scales of both juvenile and adult fish and the localization and signal intensity varied with the species and age of
the animals. The ERs may be co-localized in cells of the scale posterior region that expressed tartrateresistant acid phosphatase (TRAP), a marker for osteoclasts. These results suggest that the calcium mobilizing
action of 17b-estradiol on fish scales is via its direct action on ERs localized in osteoclasts.
Descrição
Palavras-chave
Estrogen receptor Estradiol Calcium Osteoclast Esmaloid scale Teleost
Contexto Educativo
Citação
Pinto, P. I. S.; Estevao, M. D.; Redruello, B.; Socorro, S. M.; Canario, A. V. M.; Power, D. M.Immunohistochemical detection of estrogen receptors in fish scales, General and Comparative Endocrinology, 160, 1, 19-29, 2009.
Editora
Elsevier