FCT2-Artigos (em revistas ou actas indexadas)
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Percorrer FCT2-Artigos (em revistas ou actas indexadas) por Objetivos de Desenvolvimento Sustentável (ODS) "09:Indústria, Inovação e Infraestruturas"
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- Advanced nanotherapeutic strategies transforming diabetic wound healingPublication . Ramos, Filipa; Kumar, Girish; Virmani, Tarun; Sharma, Abhishek; Duarte, Sofia O. D.; Fonte, PedroDue to their high recurrence rates and slow healing, diabetic wounds are becoming a greater public health concern [Citation1]. Each year, 1.6 million cases of diabetic wounds occur in the United States alone, affecting approximately 18.6 million people worldwide [Citation2]. Because of poor cellular regeneration, increased inflammation, and reduced angiogenesis, traditional treatments like debridement, antibiotics, and dressings usually do not work [Citation3]. To overcome the limitations of traditional treatments, there is now a significant demand for advanced therapeutic modalities that promise accurate, efficient, and rapid healing processes [Citation4]. These include microneedles (MNs), exosomes, tetrahedral framework nucleic acids (tFNAs), three-dimensional scaffolds, gene therapy, oxygen-releasing biomaterials, phototherapies, and nanozymes.
- Another tool for chondrichthyan ex situ conservation: first-time chimaera monstrosa sperm cryopreservationPublication . García-Salinas, Pablo; Gallego, Victor; Asturiano, Juan F.Chondrichthyans, which comprises elasmobranchs (sharks and rays) and holocephalans (chimaeras), are one of the most endangered group of vertebrates on the planet. Ex situ conservation programs, such as captive breeding, are tools that can be used to improve the status of some of the most sensitive species belonging to these groups. However, the use of reproductive techniques is necessary when planning sustainable breeding programs in controlled environments. In recent years, our group has described the protocols necessary to achieve viable sperm extraction and its cryopreservation in 13 species. However, the use of these techniques in the holocephalan group has not yet been explored. Here, the process of obtaining viable sperm in a holocephalan species, Chimaera monstrosa, is presented for the first time. The sperm was obtained from animals recovered from bottom trawling bycatch. It was possible to recover sperm from both males (n = 3), using cannulation and abdominal massage, and females (n = 2), by directly accessing their oviductal glands. Sufficient sperm was obtained from the males to apply cryopreservation protocols developed for elasmobranchs. For this purpose, the sperm was diluted in an extender for elasmobranchs (1 sperm:9 extender) previously developed by our group. The cryopreservation of sperm was achieved through the addition of different cryoprotectants to the extender: methanol, dimethyl sulfoxide (DMSO) and fresh egg yolk. Samples were frozen inside a Styrofoam box using vapor of liquid nitrogen and preserved in liquid nitrogen. Sperm quality was assessed by studying motility and membrane integrity post-thawing. The initial motility and membrane integrity values were close to 54%. The best post-thawing motility values were obtained with a combination of 5% DMSO, 5% methanol and 10% egg yolk, which induced motility values close to 25% and membrane integrity values close to 24%. This is the first time that sperm from this group of animals has been cryopreserved, expanding our knowledge on their reproductive biology and the tools available for their conservation.
- Bridging the ESG data gap: transparent metrics and rankings for emerging financial marketsPublication . QACHACH, AZHAR RIM; El Mahrad, Badr; Kharbouch, Omar; Moumen, Aniss; Aoufi, Sara El; Gueddari, Manal El; Abdallah-Ou-Moussa, SoukainaEnvironmental, Social, and Governance (ESG) performance has become a pivotal driver of firm valuation, investment flows, and capital market stability and a critical dimension of corporate sustainability and investor decision-making. Yet, emerging markets face structural barriers to standardized ESG measurement due to limited data availability and inconsistent disclosures. This study addresses this gap by developing a simplified, transparent and indicator-based ESG assessment model tailored to the Moroccan capital market using publicly available data from 20 companies listed in the MASI ESG Index on the Casablanca Stock Exchange. The framework evaluates 12 equally weighted indicators across environmental, social, and governance pillars, and employs the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS), a Multi-Criteria Decision-Making (MCDM) method, to generate firm-level ESG scores and rankings. In addition to equal-weighted rankings, the model was stress-tested using entropy-based and expert-informed weights. Results reveal a wide disparity in ESG maturity: while environmental reporting is relatively advanced, social and governance disclosures lag behind. Top-ranking firms align closely with international frameworks such as GRI, whereas others lack fundamental transparency. By offering a replicable, low-data ESG scoring method applicable to other emerging markets, this research provides actionable insights for investors, regulators, and corporate leaders. The findings contribute to the financial literature on ESG integration, support the design of sustainable investment strategies, and advance policy efforts to strengthen capital market resilience across the MENA region.
- Combining adequate BRUV deployment times with individual photo-identification improves monitoring of shark populations in the CaribbeanPublication . Kohler, Johanna; Gore, Mauvis; Ormond, Rupert; Mason, Katherine; Veeder, Anne; Austin, TimothySharks play a key role in coral reef ecosystems, but Caribbean populations are concerningly low. When monitoring endangered species, it is critical to use minimally invasive tools and protocols that are adequate for local species and the environment. This study investigated the adequate deployment time of baited remote underwater videos (BRUVs) for shark studies in the Cayman Islands and whether the use of photo-identification to recognise individuals (MaxIND) on BRUVs could im prove abundance estimates (in comparison to MaxN) and the analysis of shark behaviour. From 2015 to 2018, a total of 557 BRUVs were deployed with recording times ranging from 3.8 to 211.03 min. The results showed that (1) of the total number of individual sharks recorded on videos, 95% of individuals were recorded within the first 110 min (slight variations between species), (2) MaxIND values were 1.1-1.5 times greater than that of MaxN (ratios varying with species) and (3) time of first arrival (Tarrive) was similar for all recorded species but time spent in front of the camera's field of view (Tvisit) and activity levels (count of entries in camera's field of view) varied between species. The results provide key information to improve the localised monitoring of rare/endangered species and can inform conservation management.
- Customising sustainable bio-based polyelectrolytes: introduction of charged and hydrophobic groups in cellulosePublication . Magalhães, Solange; Aliaño González, María José; Cruz, Pedro F.; Rosenberg, Rose; Haffke, Dirk; Norgren, Magnus; Alves, Luís; Medronho, Bruno; Rasteiro, Maria da GraçaAbstract: Cellulose has been widely explored as a sustainable alternative to synthetic polymers in industrial applications, thanks to its advantageous properties. The introduction of chemical modifications on cellulose structure, focusing on cationic and hydrophobic modifications, can enhance its functionality and expand the range of applications. In the present work, cationization was carried out through a two-step process involving sodium periodate oxidation followed by a reaction with the Girard T reagent, yielding a degree of substitution for cationic groups (DScationic) between 0.3 and 1.8. Hydrophobic modification was achieved via esterification with fatty acids derived from commercial plant oils, using an enzyme-assisted, environmentally friendly method. Lipase-catalysed hydrolysis, optimised at 0.25% enzyme concentration and with a 1 h reaction time, produced an 84% yield of fatty acids, confirmed by FTIR and NMR analyses. The degree of substitution for hydrophobic groups (DShydrophobic) ranged from 0.09 to 0.66. The molecular weight (MW) of the modified cellulose derivatives varied from 1.8 to 141 kDa. This dual modification strategy enables the creation of cellulose-based polymers with controlled electrostatic and hydrophobic characteristics, customisable for specific industrial applications. Our approach presents a sustainable and flexible solution for developing cellulose derivatives tailored to diverse industrial needs.
- Daniel H. Burckhardt at 70—an appreciationPublication . Ouvrard, David; Serbina, Liliya Štarhová; Aguiar, António; Drohojowska, Jowita; Halbert, Susan E.; Raman, Anantanarayanan; Sharma, Anamika; Maseko, Zolile; Mauda, Evans; Zarcos Duarte, Beatriz; Cho, Geonho; Rendón-Mera, Diana Isabel; Yang, Man-Miao; Percy, Diana M.; Zürcher-Pfander, Isabelle; Malenovský, Igor; Bastin, Saskia; Queiroz, Dalva L.PD Dr Daniel H. Burckhardt (Figs 1–4) turned 70 on 26 July 2023.His extensive body of work on the taxonomy of insects, initiated even before his doctoral thesis on Western Palaearctic Aphalaridae in 1983, has since expanded to encompass all major psyllid groups (Hemiptera: Sternorrhyncha: Psylloidea), both extant and extinct, but also moss bugs (Hemiptera: Coleorrhyncha) and several families of beetles (Coleoptera, mainly Passandridae, but also Jacobsoniidae, Pterogeniidae and Staphylinidae). These contributions have had a profound and lasting impact on our global understanding of these relatively small and often overlooked groups of insects. The biographical details of Daniel Burckhardt’s career and life will not be revisited here, as they have already been thoroughly documented elsewhere. In terms of his tireless dedication and remarkable productivity, the comprehensive list of his publications at the end of this article speaks for itself.Another telling measure of his influence within entomology is the number of PhD students he has mentored, formally or informally, many of whom have gone on to establish successful careers in leading research institutions and natural history museums across Europe and beyond. At a time when taxonomic expertise is rapidly declining, with serious implications for biodiversity research, agriculture and conservation, his intellectual legacy, marked by rigour, precision, and clarity, has been effectively passed on to the next generation. This tribute aims above all to highlight the personal and professional impact Daniel Burckhardt has had on so many colleagues and collaborators. Rather than a conventional biography, it offers a space for those who have worked with him to share their experiences and anecdotes, and to reflect on the influence he has had on their scientific journeys.
- The de sitter swampland conjectures in the context of chaplygin-inspired inflationPublication . Bertolami, Orfeu; Potting, Robertus; Sá, PauloIn this work, we discuss the de Sitter swampland conjectures in the context of the generalized Chaplygin-inspired inflationary model. We demonstrate that these conjectures can be satisfied, but only in the region of the parameter space far away from the General Relativity limit. The cosmic microwave background data had already been found to restrict the allowed inflationary potentials of this model. Our results impose a further limitation on the possible potentials.
- Edible coatings enhance storability and preserve quality of kiwiberry (Actinidia arguta L.) cv. Ken’s RedPublication . Guerreiro, Adriana; Gago, Custódia; Passos, Dário; Martins, Jaime; Cruz, Sandra P.; Guerra, Rui; Veloso, Fernão; Antunes, Maria DulceKiwiberries, an emerging fruit variety with increasing consumer demand, face significant commercialization challenges due to their short shelf life. This study evaluates the effectiveness of edible alginate-based coatings in extending the shelf life of kiwiberries. Two emulsion types—coarse (Coarse) and nanoemulsions (Nano)—and two application methods—spraying (Spray) and dipping (Dip)—were tested. Additionally, the use of visible/near-infrared (Vis-NIR) spectroscopy for non-destructive quality monitoring was explored. Coatings were prepared with 2% (w/v) alginate (SAlg) enriched with eugenol (Eg) and citral (Ct) at their minimum inhibitory concentrations (MICs) of 0.10% and 0.15%, respectively, as well as at double these concentrations. This resulted in a total of ten different treatments. The fruits were stored at approximately 5 ◦C, with evaluations conducted after 5 days, 2 weeks, and 4 weeks. Quality parameters, including color, firmness, soluble solid content (SSC), dry matter, weight loss, decay, total phenolic content (TPC), flavonoids, DPPH, FRAP, and malondialdehyde (MDA) levels, were analyzed. Over the storage period, color and SSC increased, while firmness decreased. Weight loss and MDA levels showed the most significant changes, particularly in treatments with higher essential oil concentrations. Notably, treatments such as SAlg Spray, SAlg Dip, SAlg Ct 0.15 + Eg 0.1 Nano Spray, SAlg Ct 0.15 + Eg 0.1 Nano Dip, and SAlg Ct 0.15 + Eg 0.1 Coarse Dip demonstrated superior preservation of kiwiberry quality. Moreover, Vis-NIR spectroscopy proved valuable for distinguishing between coating treatments, highlighting its potential for non-destructive quality assessment.
- Enhancing polycaprolactone with levulinic acid-extracted lignin: toward sustainable bio-based polymer blendsPublication . Melro, Elodie; Duarte, Hugo; Antunes, Filipe E.; Valente, Artur J. M.; Romano, Anabela; Medronho, BrunoThe growing demand for sustainable materials has intensified the search for biodegradable polymers. Poly(ε-caprolactone) (PCL), though biodegradable, is fossil-derived. In this study, a novel lignin extracted from pine wood using a green solvent was incorporated into PCL and compared with commercial lignins (dealkaline, alkaline, and lignosulfonate). The lignin additions imparted antioxidant properties, enhanced thermal stability, and promoted circular economy goals through lignin valorization. Notably, the green-extracted lignin showed superior compatibility with PCL when compared with commercial lignins, as evidenced by lower water uptake and solubility, and improved surface hydrophobicity (higher contact angle). Although the addition of lignin reduced the tensile strength and elongation at break, it greatly increased the PCL radical scavenging activity (DPPH) from 8 ± 1% of neat PCL to 94.8 ± 0.3% when 20 wt% of lignin-LA was added. Among the tested lignins, lignin-LA stands out as the most promising candidate to be applied as a functional additive in biodegradable polymer blends and composites for advanced sustainable applications. Not only given its intrinsically higher sustainability but also due to its capacity for improving the thermal properties of PCL–lignin blends.
- Flavobacterium magnesitis sp. nov. and Flavobacterium zubiriense sp. nov. two novel Flavobacterium species isolated from alkaline magnesite residuesPublication . Matos, Leonor; Maccarrio, Lorrie; Chung, Ana Paula; Proença, Diogo Neves; Sørensen, Søren; Morais, Paula V; Francisco, RomeuThree bacterial strains, designated FZUC8N2.13, FBOR7N2.3 and FZUR7N2.5, were isolated from distinct magnesite residues in Spain. Phylogenetic and phylogenomic analysis places them within the genus . Strains FBOR7N2.3 and FZUR7N2.5 share 100% of similarity in the 16S rRNA gene sequence, and both are most closely related to AR-3-4 with which they share 97.5% of 16S rRNA gene similarity. Strain FZUC8N2.13 forms a distinct lineage most closely related to IMCC36792 with 97.7% 16S rRNA gene similarity. The closest phylogenomic neighbours of these three strains are DSM 1076, '' BBQ-18 and PK15. The average nucleotide identity and digital DNA-DNA hybridization values between the three strains and closest members of the genus are below the threshold values of 95% and 70%, respectively. Strains FZUC8N2.13, FBOR7N2.3 and FZUR7N2.5 stain Gram-negative, are rod-shaped and form yellow colonies. Optimum growth occurs at 25 °C and pH 7. The genomic G+C contents are 33.4 mol% for strain FZUC8N2.13 and 33.2 mol% for strains FBOR7N2.3 and FZUR7N2.5. The major isoprenoid quinone is menaquinone 6. The major fatty acids are summed feature 3 (C ω7c and/or C ω6c) (22.6-31.1%), iso-C (13.6-16.2 %) and anteiso-C (8.7-10.5%). The polar lipids consist of two aminolipids, two aminophospholipids and one glycolipid. The phylogenetic, phylogenomic, phenotypic and chemotaxonomic data indicate that FZUC8N2.13, FBOR7N2.3 and FZUR7N2.5 are distinct from the described species of and should be classified as novel species, for which we propose the names for strain FZUC8N2.13 (=UCCCB 179=CECT 30977) and for strains FBOR7N2.3 (=UCCCB 178=CECT 30976) and FZUR7N2.5 (=UCCCB 216=CECT 31036).