I. Componente Universitária
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- 1,1-Bis(Phenylseleno)-and 1,1-Bis(Methylseleno)- alkyllithiums as building-blocks in organic synthesisPublication . Vanende, D.; Cravador, A.; Krief, A.1,1 - Bis(phenylseleno)alkyllithiums are conveniently prepared from the corresponding selenoacetals and lithium tetramethylpiperidide in HMPT/THF.
- 1,2,4-Trioxolane and 1,2,4,5-Tetraoxane endoperoxides against old-world Leishmania parasites: in vitro activity and mode of actionPublication . Mendes, Andreia; Armada, Ana; Cabral, Lília; Amado, Patrícia; Campino, Lenea; Cristiano, Maria de Lurdes; Cortes, SofiaLeishmaniasis remains one of the ten Neglected Tropical Diseases with significant morbidity and mortality in humans. Current treatment of visceral leishmaniasis is difficult due to a lack of effective, non-toxic, and non-extensive medications. This study aimed to evaluate the selectivity of 12 synthetic endoperoxides (1,2,4-trioxolanes; 1,2,4,5-tetraoxanes) and uncover their biochemical effects on Leishmania parasites responsible for visceral leishmaniasis. The compounds were screened for in vitro activity against L. infantum and L. donovani and for cytotoxicity in two monocytic cell lines (J774A.1 and THP-1) using the methyl thiazol tetrazolium assay. Reactive oxygen species formation, apoptosis, and mitochondrial impairment were measured by flow cytometry. The compounds exhibited fair to moderate anti-proliferative activity against promastigotes of the 2 Leishmania species, with IC50 values ranging from 13.0 ± 1.7 µM to 793.0 ± 37.2 µM. Tetraoxanes LC132 and LC138 demonstrated good leishmanicidal activity on L. infantum amastigotes (IC50 13.2 ± 5.2 and 23.9 ± 2.7 µM) with low cytotoxicity in mammalian cells (SIs 22.1 and 118.6), indicating selectivity towards the parasite. Furthermore, LC138 was able to induce late apoptosis and dose-dependent oxidative stress without affecting mithocondria. Compounds LC132 and LC138 can be further explored as potential antileishmanial chemotypes.
- 1-Methylcyclopropene and lemongrass essential oil nanocoatings effect on the preservation of cold stored ‘Rocha’ pearPublication . Gago, Custódia; Guerreiro, Adriana; Cruz, Sandra; Martins, Nuno; Cabrita, Maria João; Miguel, Maria; Faleiro, Maria Leonor; Antunes, Maria DulceThe effects of coating 'Rocha' pear with alginate-based nanoemulsions enriched with lemongrass essential oil (LG) was evaluated and compared to the usual 1-MCP treatment. Fruit were treated with 1-MCP (312 nL L-1) or coated with nanoemulsions: sodium alginate 2 % (w/w) + lemongrass essential oil 1.25 % (w/w) (LG 1.25 %) or lemongrass essential oil 2.5 % (w/w) (LG 2.5 %). Then, fruit were stored at 0 degrees C and 90-95 % relative humidity (RH), for eight months. Fruit samples were collected at harvest and after two, four, six and eight months of cold storage, and then transferred to shelf-life at 22 degrees C. Upon removal and after 7 d shelf-life, fruit symptoms of superficial scald and internal browning, ethylene production, color CIE (L*, hue), firmness, soluble solids content (SSC), titratable acidity (TA), weight loss, electrolytic leakage (EL), antioxidant activity and fatty acids of pear peel, microbial growth and sensory analyses were evaluated. Coatings and 1-MCP reduced fruit color evolution and preserved better firmness than control. Coatings and 1-MCP did not affect SSC and TA. Treatments did not influence the sensory quality. Microbial growth was within the safety limits in all treatments. Treatments with 1-MCP and LG-nanoemulsions were similarly efficient to reduce superficial scald, nevertheless the LG-nanoemulsions showed higher internal disorders after 8 months of storage and LG 2.5 % had higher decay at the same period, similar to control. 1-MCP treated fruit had the lowest softening rate after shelf-life up to 4 months and LG 2.5 % showed higher weight loss. Also, ethylene production was higher in control and LG 1.25 % up to 6 months plus shelf-life, while after 8 months there was no difference among treatments. This study suggests that 1-MCP is the most efficient for preserving quality of 'Rocha' pear for 8 months, while up to 6 months the best effect is obtained with LG 1.25 % nanocoatings.
- 10 Anos da Medievalista: as revistas de ciências sociais e os seus desafiosPublication . Sousa, Bernardo Vasconcelos e; Oliveira, Luís FilipeNo dia 25 de Setembro de 2015, teve lugar na FCSH-UNL, o seminário Internacional "As Revistas de Ciências Sociais e os seus Desafios", para assinalar os dez anos de publicação ininterrupta da Medievalista OnLine. Tal como constava no desdobrável de divulgação, pretendia-se que a iniciativa fosse mais do que uma celebração e criasse a oportunidade para se fazer um balanço crítico, reflectindo “em conjunto com outras revistas, sobre os problemas e os desafios que hoje se colocam às publicações periódicas na área da História e das Ciências Sociais”.
- 100 anos da revista OrpheuPublication . Carmo, Carina Infante doNeste artigo revê-se dos aspectos mais significativos protagonizados pela revista Orpheu, no quadro da modernidade literária e política, e seus impactos mais relevantes na literatura portuguesa subsequente.
- 108P PICTuRE: Real-world treatment pathways in stage III non-small cell lung cancer in PortugalPublication . Teixeira, M.E.; Oliveira Fernandes, M.G.; Ferreira, M.L.S.; Estevinho, F.; Lopes, J.A.; Araújo, A.; Pacheco Figueiredo, M.M.; Barradas Lopes, M.L.; Lopes, M.; Brito, U.; Barroso, A.M.P.; Camacho, C.; Parente, B.; Felizardo, M.M.; Meleiro, A.; Bernardo, M.; Bernardo, F.; Figueiredo, S.Stage III NSCLC is a heterogeneous and complex setting that requires multimodal management. Recent advances in modern medicine led to the implementation of immuno-oncology (IO) into clinical practice. PICTuRE aims to assess the clinical management and the IO impact on clinical outcomes in stage III NSCLC patients (pts).
- 1867/8: a odisseia submarina de Jules VernePublication . Carvalho, Ana Alexandra Seabra deDe todos os escritores franceses, Jules Verne será talvez o mais conhecido e reconhecido fora do seu país. Pai – com H. G. Wells – da ficção científica, segundo uns; ou mero autor de livros para a juventude, segundo outros, ele revela-se, na verdade, um mestre da narrativa de aventuras, de divulgação científica e de antecipação. Neste estudo, começaremos por uma síntese dos aspetos principais da sua vastíssima obra; em seguida, recordaremos um dos seus mais afamados romances: Vinte Mil Léguas Submarinas.
- A 1ª. República na intervenção sonâmbula de José Gomes FerreiraPublication . Carmo, Carina Infante doReveladora de uma forte consciência histórica, a obra autobiográfica de José Gomes Ferreira (1900-1985) elege I República como matriz do século XX português, ciente, em todo o caso, que lhe cabe sobretudo escrever «para a eternidade efémera da História dos Momentos Ignorados». Em A Memória das Palavras (1965) e Revolução Necessária (1975), é particularmente notória a forma como situa a raiz do fascínio revolucionário no espaço nacional e lisboeta da sua aprendizagem infantil, em detrimento do exemplo mais previsível, a Revolução Russa de 1917. Dessa forma assume a filiação jacobina das barricadas, sedimentada nas leituras literárias do romantismo social e na admiração pelo pai, que foi político republicano e maçónico empenhado. Ao fazer da História matéria autobiográfica, José Gomes Ferreira não apenas recorta a formação pessoal dentro da história familiar e colectiva. Também alinha deliberadamente na valorização ideológica da I República e, por extensão, do liberalismo oitocentista levada a cabo, desde os anos 40, no campo da Historiografia e dos Estudos Literários, para responder ao anátema lançado pelo salazarismo sobre esses períodos históricos. Gomes Ferreira exerce, portanto, a resistência no campo cultural por meio do exercício de memória na primeira pessoa. Quando chega o 25 de Abril (a que chama II República), tempera o entusiasmo de o viver com a lucidez da sua já longa viagem pelo século, sempre à espera de voltar a presenciar a vibração livre da multidão nas ruas. Pressente o esquecimento progressivo da I República em benefício de um novo marco revolucionário, sabendo-se testemunha exaltada e melancólica de revoluções, da sua força construtiva e dos seus desfechos magoados.
- 1st METECH workshop – From deep-sea to coastal zones: Methods and Techniques for studying PalaeoenvironmentsPublication . Veiga-Pires, Cristina; Guillaume St-OngeReconstructing past climate and past ocean circulation demands the highest possible precision and accuracy which urges the scientific community to look at different sediment records such as the ones from coastal zones to deep-sea with a more complete set of technical and methodological tools. However, the information given by each tool varies in precision, accuracy and in significance according to their environmental settings. It is therefore essential to compare tools. With that in mind, and as part of the International year of Planet Earth, a workshop entitled `From deep-sea to coastal zones: Methods and Techniques for studying palaeoenvironments' took place in Faro (Portugal), from 25–29 February 2008.
- 2-D difference gel electrophoresis approach to assess protein expression profiles in Bathymodiolus azoricus from Mid-Atlantic Ridge hydrothermal ventsPublication . Company, Rui; Antúnez, Oreto; Bebianno, Maria João; Cajaraville, Miren P.; Torreblanca, AmparoHydrothermal vent mussels Bathymodiolus azoricus are naturally exposed to toxic chemical species originated directly from vent chimneys. The amount of toxic elements varies significantly among vent sites along the Mid-Atlantic Ridge and B. azoricus must be able to adapt to changes in hydrothermal fluid composition, temperature and pressure. The aim of this work was to study changes in the proteome in the "gill-bacteria complex" of mussels B. azoricus from three hydrothermal vent sites with distinct environmental characteristics using 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE). Results showed that 31 proteins had different expression profiles among vent sites and both cluster and principal component analysis confirm a clear separation of mussels between sites. This suggests the existence of specific parameters grouping individuals from the same hydrothermal site. Protein spots of the more abundant differentially expressed proteins were excised, digested with trypsin and identified by mass spectrometry. All identified proteins (actin, ubiquinone, S-adenosylhomocysteine hydrolase, cysteine peptidases, chaperonin and catalase) have been related previously with oxidative stress conditions and are known to be affected by ROS inducing stressors, including metals. Results point out to specific adaptations at the proteome level of B. azoricus depending on the level of toxicants present in their environment.
- 2018 EULAR recommendations for physical activity in people with inflammatory arthritis and osteoarthritisPublication . Osthoff, Anne-Kathrin Rausch; Niedermann, Karin; Braun, Juergen; Adams, Jo; Brodin, Nina; Dagfinrud, Hanne; Duruoz, Tuncay; Esbensen, Bente Appel; Guenther, Klaus-Peter; Hurkmans, Emailie; Juhl, Carsten Bogh; Kennedy, Norelee; Kiltz, Uta; Knittle, Keegan; Nurmohamed, Michael; Pais, Sandra; Severijns, Guy; Swinnen, Thijs Willem; Pitsillidou, Irene A.; Warburton, Louise; Yankov, Zhivko; Vlieland, Theodora P. M. VlietRegular physical activity (PA) is increasingly promoted for people with rheumatic and musculoskeletal diseases as well as the general population. We evaluated if the public health recommendations for PA are applicable for people with inflammatory arthritis (iA; Rheumatoid Arthritis and Spondyloarthritis) and osteoarthritis (hip/knee OA) in order to develop evidence-based recommendations for advice and guidance on PA in clinical practice. The EULAR standardised operating procedures for the development of recommendations were followed. A task force (TF) (including rheumatologists, other medical specialists and physicians, health professionals, patient-representatives, methodologists) from 16 countries met twice. In the first TF meeting, 13 research questions to support a systematic literature review (SLR) were identified and defined. In the second meeting, the SLR evidence was presented and discussed before the recommendations, research agenda and education agenda were formulated. The TF developed and agreed on four overarching principles and 10 recommendations for PA in people with iA and OA. The mean level of agreement between the TF members ranged between 9.8 and 8.8. Given the evidence for its effectiveness, feasibility and safety, PA is advocated as integral part of standard care throughout the course of these diseases. Finally, the TF agreed on related research and education agendas. Evidence and expert opinion inform these recommendations to provide guidance in the development, conduct and evaluation of PA-interventions and promotion in people with iA and OA. It is advised that these recommendations should be implemented considering individual needs and national health systems.
- 2019 EULAR points to consider for non-physician health professionals to prevent and manage fragility fractures in adults 50 years or olderPublication . Adams, Jo; Wilson, Nicky; Hurkmans, Emalie; Bakkers, Margot; Balážová, Petra; Baxter, Mark; Blavnsfeldt, Anne-Birgitte; Briot, Karine; Chiari, Catharina; Cooper, Cyrus; Dragoi, Razvan Gabriel; Gäbler, Gabriele; Lems, Willem; Mosor, Erika; Pais, Sandra; Simon, Cornelia; Studenic, Paul; Tilley, Simon; de la Torre-Aboki, Jenny; Stamm, Tanja AObjective To establish European League Against Rheumatism (EULAR) points to consider for non-physician health professionals to prevent and manage fragility fractures in adults 50 years or older. Methods Points to consider were developed in accordance with EULAR standard operating procedures for EULAR-endorsed recommendations, led by an international multidisciplinary task force, including patient research partners and different health professionals from 10 European countries. Level of evidence and strength of recommendation were determined for each point to consider, and the mean level of agreement among the task force members was calculated. Results Two overarching principles and seven points to consider were formulated based on scientific evidence and the expert opinion of the task force. The two overarching principles focus on shared decisions between patients and non-physician health professionals and involvement of different non-physician health professionals in prevention and management of fragility fractures. Four points to consider relate to prevention: identification of patients at risk of fracture, fall risk evaluation, multicomponent interventions to prevent primary fracture and discouragement of smoking and overuse of alcohol. The remaining three focus on management of fragility fractures: exercise and nutritional interventions, the organisation and coordination of multidisciplinary services for post-fracture models of care and adherence to anti-osteoporosis medicines. The mean level of agreement among the task force for the overarching principles and the points to consider ranged between 8.4 and 9.6. Conclusion These first EULAR points to consider for non-physician health professionals to prevent and manage fragility fractures in adults 50 years or older serve to guide healthcare practice and education.
- 24-hour rhythmicity of seizures in refractory focal epilepsyPublication . Nzwalo, Hipólito; Cordeiro, Inês Menezes; Santos, Ana Catarina; Peralta, Rita; Paiva, Teresa; Bentes, CarlaThe occurrence of seizures in specific types of epilepsies can follow a 24-hour nonuniform or nonrandom pattern. We described the 24-hour pattern of clinical seizures in patients with focal refractory epilepsy who underwent video-electroencephalography monitoring. Only patients who were candidates for epilepsy surgery with an unequivocal seizure focus were included in the study. A total of 544 seizures from 123 consecutive patients were analyzed. Specific time of seizures were distributed along 3- or 4-hour time blocks or bins throughout the 24-hour period. The mean age of the subjects was 37.7 years, with standard deviation of 11.5 years, median of 37. The majority were females (70/56%). The majority of patients had a seizure focus located in the mesial temporal lobe (102/83%) and in the neocortical temporal lobe (13/11%). The remaining patients had a seizure focus located in the extratemporal lobe (8/6%). The most common etiology was mesial temporal sclerosis (86/69.9%). Nonuniform seizure distribution was observed in seizures arising from the temporal lobe (mesial temporal lobe and neocortical temporal lobe), with two peaks found in both 3- and 4-hour bins: 10:00-13:00/16:00-19:00 and 08:00-12:00/16:00-20:00 respectively (p = 0.004). No specific 24-hour pattern was identified in seizures from extratemporal location. The 24-hour rhythmicity of seizure distribution is recognized in certain types of epilepsy, but studies on the topic are scarce. Their replication and validation is therefore needed. Our study confirms the bimodal pattern of temporal lobe epilepsy independently of the nature of the lesion. However, peak times differ between different studies, suggesting that the ambient, rhythmic exogenous factors or environmental/social zeitgebers, may modulate the 24-hour rhythmicity of seizures. Characterization of these 24-hour patterns of seizure occurrence can influence diagnosis and treatment in selected types of epilepsy, such as the case of temporal lobe epilepsy, the most common drug-resistant epilepsy. (C) 2015 Elsevier Inc. All rights reserved.
- 3 x 3 lemma for star-exact sequencesPublication . Gran, Marino; Janelidze, Zurab; Rodelo, DianaA regular category is said to be normal when it is pointed and every regular epimorphism in it is a normal epimorphism. Any abelian category is normal, and in a normal category one can define short exact sequences in a similar way as in an abelian category. Then, the corresponding 3 x 3 lemma is equivalent to the so-called subtractivity, which in universal algebra is also known as congruence 0-permutability. In the context of non-pointed regular categories, short exact sequences can be replaced with "exact forks" and then, the corresponding 3 x 3 lemma is equivalent, in the universal algebraic terminology, to congruence 3-permutability; equivalently, regular categories satisfying such 3 x 3 lemma are precisely the Goursat categories. We show how these two seemingly independent results can be unified in the context of star-regular categories recently introduced in a joint work of A. Ursini and the first two authors.
- 3D Modeling of the Milreu Roman Heritage with UAVsPublication . Rodrigues, José Inácio; Figueiredo, Mauro; Bernardes, João Pedro; Gonçalves, CésarIn this paper we present a methodology to build a 3D model of a roman heritage site in the South of Portugal, known as Milreu, covering a region of about one hectare. Today's Milreu ruins, a national heritage site, were once part of a 4rd century, luxurious villa-style manor house, which was subsequently converted into a thriving farm. Due to its relevance, it is important to make the 3D model of the Milreu ruins, to be available for the exploration in the Web and for virtual and augmented reality applications for mobile devices. This paper demonstrates the use of UAVs for the reconstruction of the 3D models of the ruins from vertical and oblique aerial photographs. To enhance the model quality and precision, terrestrial photographs were also incorporated in the workflow. This model is georeferenced, which give us the possibility to automatically determine accurate measurements of the Roman structures.
- 3D printed gluten-free cereal snack with incorporation of Spirulina (Arthrospira platensis) and/or Chlorella vulgarisPublication . Letras, Pedro Miguel Viriato; Varela, J.; Raymundo, Anabela3D food printing is a recent promising technology to break cultural barriers by introducing new food sources as microalgae, through innovative food shapes and textures, in a resource scarce world, unfeasible with the current intensive meat and agriculture industries. The present work intended to create an innovative gluten-free cereal snack nutritionally improved by incorporation of microalgae Chlorella vulgaris and Arthrospira platensis (Spirulina) using three-dimensional (3D) printing technology. Furthermore, design settings alterations were performed, registered, and presented in a poster, to explore the creative reach of this technology. From control and different percentage microalgae doughs (5-30%), the ones with most adequate rheology and texture properties for a correct printing process were selected and baked. Nutritional characterisation of the control and 5% microalgae-containing snacks was thus performed, including their total protein and fatty acid content, ashes, humidity, water activity, energy, and carbohydrates, as well as total phenolics, pigments and antioxidant activity. Physical traits of snacks including their colour and dimensions were also analysed. Control snacks presented a lighter and yellow colour compared to snacks containing Chlorella and Spirulina, which had higher green chromaticity, resulting from the natural colour of the biomass. Nutritional characterization revealed Chlorella- and Spirulina-containing snacks had both higher contents of protein and essential minerals than control snack. Overall, 5% Spirulina-containing snacks presented the most promising nutritional and sensory performance with higher antioxidant activity, mineral and protein content. 3D food printing is still limited to the built-in computer assisted design (CAD) software that printers provide. Incorporation of 5% Spirulina improved the nutritional characterization and consumer’s perception of gluten-free products incorporating microalgae. Even so, microalgae incorporation in gluten-free foods using 3D printing requires further studying to allow its commercialization in the food market, while helping to provide consumers a more sustainable diet and respond to the current scarcity of food resources.
- [4+2] Cycloadditions of 3-Tetrazolyl-1,2-diaza-1,3-butadienes: synthesis of 3-Tetrazolyl-1,4,5,6-tetrahydropyridazinesPublication . Lopes, Susana M. M.; Brigas, Amadeu; Palacios, Francisco; Lemos, A.; Melo, Teresa M. V. D. Pinho eThe synthesis of new 3-tetrazolyl-1,4,5,6-tetrahydropyridazines by Diels–Alder reactions of ethyl 3-(tetrazol-5-yl)-1,2- diaza-1,3-butadiene-1-carboxylates is reported. These 1,2- diaza-1,3-dienes reacted with electron-rich heterocycles, nucleophilic olefins and cumulenes, as well as with electrondeficient dienophiles, to give the target compounds regioselectively. Computational studies corroborated the rationalization of the observed reactivity and selectivity.
- 4,4 '-Di-tert-butyl-2,2 '-bipyridinePublication . Amarante, Tatiana R.; Figueiredo, Sonia; Lopes, Andre D.; Gonçalves, Isabel S.; Almeida Paz, Filipe A.In the title compound, C18H24N2, the molecular unit adopts a trans conformation around the central C-C bond [N-C-C-N torsion angle of 179.2(3)degrees], with the two aromatic rings almost coplanar [dihedral angle of only 0.70 (4)degrees]. The crystal packing is driven by co-operative contacts involving weak C H center dot center dot center dot N and C-H center dot center dot center dot pi interactions, and also the need to fill effectively the available space.
- 49P Impact of nutritional factors in response and survival of patients with gestational trophoblastic neoplasiaPublication . Roque Perez, K.; Galvez Nino, M.A.; Castro, M.W.; Vallejos, H.D.; Ruiz, R.E.; Valdiviezo Lama, N.I.; Coanqui Gonzales, O.; Olivera, M.; Perez, J.; Lopez, A.; Velarde, C.; De Mello, Ramon Andrade; Mas Lopez, L.A.Gestational trophoblastic neoplasia (GTN) is a rare tumor with an excellent prognosis. Despite the International Federation of Gynecology and Obstetrics (FIGO) risk score, other factors that may influence survival remain unknown.
- 5-Deazaalloxazine as photosensitizer of singlet oxygen and potential redox-sensitive agentPublication . Insińska-Rak, Małgorzata; Golczak, Anna; Gierszewski, Mateusz; Anwar, Zubair; Cherkas, Volodymyr; Kwiatek, Dorota; Sikorska, Ewa; Khmelinskii, Igor; Burdziński, Gotard; Cibulka, Radek; Mrówczyńska, Lucyna; Kolanowski, Jacek Lukasz; Sikorski, MarekFlavins are a unique class of compounds that combine the features of singlet oxygen generators and redox-dependent fluorophores. From a broad family of flavin derivatives, deazaalloxazines are significantly underdeveloped from the point of view of photophysical properties. Herein, we report photophysics of 5-deazaalloxazine (1a) in water, acetonitrile, and some other solvents. In particular, triplet excited states of 1ain water and in acetonitrile were investigated using ultraviolet-visible (UV-Vis) transient absorption spectroscopy. The measured triplet lifetimes for 1a were all on the microsecond time scale (approximate to 60 mu s) in deoxygenated solutions. The quantum yield of S-1 -> T-1 intersystem crossing for 1ain water was 0.43 based on T1 energy transfer from 1a to indicaxanthin (5) acting as acceptor and on comparative actinometric measurements using benzophenone (6). 1a was an efficient photosensitizer for singlet oxygen in aerated solutions, with quantum yields of singlet oxygen in methanol of about 0.76, compared to acetonitrile similar to 0.74, dichloromethane similar to 0.64 and 1,2-dichloroethane similar to 0.54. Significantly lower singlet oxygen quantum yields were obtained in water and deuterated water (Phi(Delta)similar to 0.42 and 0.44, respectively). Human red blood cells (RBC) were used as a cell model to study the antioxidant capacity in vitro and cytotoxic activity of 1a. Fluorescence-lifetime imaging microscopy (FLIM) data were analyzed by fluorescence lifetime parameters and distribution for different parts of the emission spectrum. Comparison of multidimensional fluorescent properties of RBC under physiological-like and oxidative-stress conditions in the presence and absence of 1a suggests its dual activity as probe and singlet-oxygen generator and opens up a pathway for using FLIM to analyze complex intracellular behavior of flavin-like compounds. These new data on structure-property relationship contribute to the body of information required for a rational design of flavin-based tools for future biological and biochemical applications.